Department of Entomology, Texas A&M University, College Station, TX.
Departamento de Biologia, Universidad del Valle de Guatemala, Guatemala.
J Med Entomol. 2020 Feb 27;57(2):649-652. doi: 10.1093/jme/tjz210.
The use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. In this study, we evaluated isotopic marking of Aedes aegypti (L.) (Diptera: Culicidae) in a laboratory setting. We determined the optimal dosage for marking adult Ae. aegypti mosquitoes with 13C and 15N. Additionally, Ae. aegypti mosquitoes were single and dually marked with 13C and 15N for up to 60 d postemergence without changes to adult body size or transgenerational marking. This report adds to the growing literature that explores the use of alternative marking methods for ecological and vector biology studies.
利用稳定同位素标记来标记蚊子为研究病媒物种的生物学特性提供了一种工具。在本研究中,我们在实验室环境下评估了对埃及伊蚊(双翅目:蚊科)进行同位素标记的效果。我们确定了用 13C 和 15N 对成年埃及伊蚊进行标记的最佳剂量。此外,埃及伊蚊在出蚊后最多 60 天内进行单重和双重 13C 和 15N 标记,而对成蚊体型或跨代标记没有影响。本报告补充了越来越多的关于探索替代标记方法用于生态和病媒生物学研究的文献。