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实验室饲养的埃及伊蚊(双翅目:蚊科)的稳定同位素标记。

Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae).

机构信息

Department of Entomology, Texas A&M University, College Station, TX.

Departamento de Biologia, Universidad del Valle de Guatemala, Guatemala.

出版信息

J Med Entomol. 2020 Feb 27;57(2):649-652. doi: 10.1093/jme/tjz210.

DOI:10.1093/jme/tjz210
PMID:31751467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7044722/
Abstract

The use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. In this study, we evaluated isotopic marking of Aedes aegypti (L.) (Diptera: Culicidae) in a laboratory setting. We determined the optimal dosage for marking adult Ae. aegypti mosquitoes with 13C and 15N. Additionally, Ae. aegypti mosquitoes were single and dually marked with 13C and 15N for up to 60 d postemergence without changes to adult body size or transgenerational marking. This report adds to the growing literature that explores the use of alternative marking methods for ecological and vector biology studies.

摘要

利用稳定同位素标记来标记蚊子为研究病媒物种的生物学特性提供了一种工具。在本研究中,我们在实验室环境下评估了对埃及伊蚊(双翅目:蚊科)进行同位素标记的效果。我们确定了用 13C 和 15N 对成年埃及伊蚊进行标记的最佳剂量。此外,埃及伊蚊在出蚊后最多 60 天内进行单重和双重 13C 和 15N 标记,而对成蚊体型或跨代标记没有影响。本报告补充了越来越多的关于探索替代标记方法用于生态和病媒生物学研究的文献。

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本文引用的文献

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Marking mosquitoes in their natural larval sites using H-enriched water: a promising approach for tracking over extended temporal and spatial scales.使用富含氢的水在其自然幼虫栖息地标记蚊子:一种在长时间和大空间尺度上进行追踪的有前景的方法。
Methods Ecol Evol. 2019 Aug;10(8):1274-1285. doi: 10.1111/2041-210x.13210. Epub 2019 May 17.
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Laboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies.实验室评估稳定同位素标记致倦库蚊(双翅目:蠓科)成虫扩散研究。
Parasit Vectors. 2019 Aug 22;12(1):411. doi: 10.1186/s13071-019-3671-9.
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Emerging and Reemerging Aedes-Transmitted Arbovirus Infections in the Region of the Americas: Implications for Health Policy.
美洲地区出现和再现的伊蚊传播虫媒病毒感染:对卫生政策的影响。
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Mosquito-Borne Human Viral Diseases: Why ?蚊媒人类病毒病:为何?
Am J Trop Med Hyg. 2018 Jun;98(6):1563-1565. doi: 10.4269/ajtmh.17-0866. Epub 2018 Mar 15.
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The importance of being urgent: The impact of surveillance target and scale on mosquito-borne disease control.紧急情况的重要性:监测目标和规模对蚊媒疾病控制的影响。
Epidemics. 2018 Jun;23:55-63. doi: 10.1016/j.epidem.2017.12.004. Epub 2017 Dec 16.
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PLoS Negl Trop Dis. 2017 Jan 30;11(1):e0005347. doi: 10.1371/journal.pntd.0005347. eCollection 2017 Jan.
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Using Stable Isotopes of Carbon and Nitrogen to Mark Wild Populations of Anopheles and Aedes Mosquitoes in South-Eastern Tanzania.利用碳和氮的稳定同位素标记坦桑尼亚东南部按蚊和伊蚊的野生种群。
PLoS One. 2016 Jul 8;11(7):e0159067. doi: 10.1371/journal.pone.0159067. eCollection 2016.
8
Dispersal of adult culex mosquitoes in an urban west nile virus hotspot: a mark-capture study incorporating stable isotope enrichment of natural larval habitats.在城市西尼罗河病毒热点地区成年库蚊的扩散:一项结合了天然幼虫栖息地稳定同位素富集的标记-捕获研究。
PLoS Negl Trop Dis. 2014 Mar 27;8(3):e2768. doi: 10.1371/journal.pntd.0002768. eCollection 2014 Mar.
9
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The global distribution and burden of dengue.登革热的全球分布和负担。
Nature. 2013 Apr 25;496(7446):504-7. doi: 10.1038/nature12060. Epub 2013 Apr 7.