Section of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Nat Struct Mol Biol. 2019 Sep;26(9):766-770. doi: 10.1038/s41594-019-0278-y. Epub 2019 Aug 22.
The discovery of RNA polymerases I, II, and III opened up a new era in gene expression. Here I provide a personal retrospective account of the transformation of the DNA template, as it evolved from naked DNA to chromatin, in the biochemical analysis of transcription by RNA polymerase II. These studies have revealed new insights into the mechanisms by which transcription factors function with chromatin to regulate gene expression.
RNA 聚合酶 I、II 和 III 的发现开启了基因表达的新纪元。在这里,我提供了一个个人的回顾,描述了 DNA 模板从裸露 DNA 到染色质的转变,这是在 RNA 聚合酶 II 对转录的生化分析中演变而来的。这些研究揭示了转录因子与染色质一起调节基因表达的机制的新见解。