Qosimah Dahliatul, Aryani Dhita Evi, Beltran Ma Asuncion Guiang, Aulanni'am Aulanni'am
Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
Laboratory of Pharmacology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
Vet World. 2019 Jun;12(6):849-854. doi: 10.14202/vetworld.2019.849-854. Epub 2019 Jun 19.
Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sepsis. The study aimed to determine the method of using animal models of sepsis diabetes through a combination of streptozotocin (STZ) and infection based on biological marker parameters.
A total of 30 male Wistar rats of 2.5-3 months old weighing approximately 150-250 g body weight (BW) divided into six treatment groups with five replications per group were used in the study. Treatment A was negative control (healthy rats) and Treatment B was the positive control (with diabetes) where rats were given STZ dose at 45 mg/kg BW on day 8 intraperitoneally (IP). The blood glucose was measured on day 10, Treatment C was a positive control (bacteria), rats inoculated with with a concentration of 10 CFU/mL on day 8 given IP and observed sepsis conditions on day 10. Treatment group (D, E, and F): Rats given STZ dose at 45 mg/kg BW on day 8 by IP and measured blood glucose on day 10, then inoculated with with different concentrations of 10 CFU/mL, 10 CFU/mL, and 10 CFU/mL on the 10 day, respectively, and were later observed the condition of sepsis on day 12. Data on diabetes bacteremia were quantitative used blood glucose levels, the bacterial count, and C-reactive protein (CRP) and were analyzed using the one-way analysis of variance test with a confidence level of 95%. Physical examination (temperature and respiration) is qualitative.
Physical examination showed that all treatments had a normal temperature, an increased pulse in Groups D, E, and F and a decrease in respiratory rate in the treatment of E and F, the bacteria found in the vital organs in all groups, and CRP levels were not significantly different at all.
Animal model of diabetes sepsis can be observed through a combination of pancreas damage, and respiration, the bacteria in the vital organs.
脓毒症的特征是炎症反应失控，可由各种微生物和毒性分泌物引发。由于内皮功能受损导致器官系统功能障碍，死亡率会升高。糖尿病与脓毒症密切相关。本研究旨在基于生物标志物参数，通过链脲佐菌素（STZ）与感染相结合的方法确定脓毒症糖尿病动物模型的构建方法。
本研究共使用30只2.5 - 3月龄、体重约150 - 250 g的雄性Wistar大鼠，分为六个治疗组，每组五次重复。治疗A为阴性对照（健康大鼠），治疗B为阳性对照（糖尿病大鼠），于第8天腹腔注射45 mg/kg体重的STZ。在第10天测量血糖；治疗C为阳性对照（细菌感染），于第8天腹腔注射浓度为10⁸ CFU/mL的细菌，并于第10天观察脓毒症情况。治疗组（D、E和F）：大鼠于第8天腹腔注射45 mg/kg体重的STZ，第10天测量血糖，然后分别于第10天接种不同浓度为10⁶ CFU/mL、10⁷ CFU/mL和10⁸ CFU/mL的细菌，并于第12天观察脓毒症情况。糖尿病菌血症的数据采用血糖水平、细菌计数和C反应蛋白（CRP）进行定量分析，并使用置信水平为95%的单因素方差分析进行分析。体格检查（体温和呼吸）为定性检查。
体格检查显示，所有治疗组体温均正常，D、E和F组脉搏加快，E和F组呼吸频率降低，所有组重要器官均发现细菌，且CRP水平均无显著差异。
通过胰腺损伤、呼吸以及重要器官中的细菌情况相结合，可以观察到糖尿病脓毒症动物模型。
