Medvedeva E S, Mouzykantov A A, Baranova N B, Dramchini M A, Chernova O A, Chernov V M
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Russia.
Kazan (Volga Region) Federal University, Russia.
Data Brief. 2019 Jun 21;25:104169. doi: 10.1016/j.dib.2019.104169. eCollection 2019 Aug.
(class Mollicutes), a major contaminant of cell cultures, quickly adapts to various classes of antimicrobials, including antimicrobial peptides. The extracellular vesicles of this bacterium can play a significant role in the development of drug-resistance Chernov et al., 2018. We compared the cellular and vesicular proteomes of strains with differing susceptibility to melittin (an antimicrobial peptide from bee venom), the genomes of which we have previously sequenced. We extracted soluble proteins from cells and extracellular vesicles of the PG8R strain showing an increased resistance to melittin, and compared them with the cellular proteome and a previously obtained vesicular proteome of the original (reference) PG8B strain Chernov et al., 2014. The cellular proteome profile of the strains differing in susceptibility to melittin was determined by using two-dimensional gel electrophoresis and MALDI-TOF/TOF MS. Here we present the cellular proteins that were differentially expressed. The vesicular proteome profile was determined by using one-dimensional electrophoresis and chromatography-mass spectrometry. A list of the extracellular vesicles proteins of the melittin-resistant strain is presented here.
支原体（Mollicutes）是细胞培养中的主要污染物，它能迅速适应各类抗菌药物，包括抗菌肽。该细菌的细胞外囊泡在耐药性发展中可能起重要作用（切尔诺夫等人，2018年）。我们比较了对蜂毒抗菌肽蜂毒肽敏感性不同的菌株的细胞和囊泡蛋白质组，这些菌株的基因组我们之前已测序。我们从对蜂毒肽耐药性增强的PG8R菌株的细胞和细胞外囊泡中提取了可溶性蛋白质，并将它们与原始（对照）PG8B菌株的细胞蛋白质组和先前获得的囊泡蛋白质组进行比较（切尔诺夫等人，2014年）。通过二维凝胶电泳和基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF MS）确定了对蜂毒肽敏感性不同的菌株的细胞蛋白质组图谱。在此我们展示了差异表达的细胞蛋白质。通过一维电泳和色谱-质谱联用确定了囊泡蛋白质组图谱。这里列出了对蜂毒肽耐药的菌株的细胞外囊泡蛋白质清单。
