Sato Jun, Inage Kazuhide, Miyagi Masayuki, Sakuma Yoshihiro, Yamauchi Kazuyo, Suzuki Masahiko, Koda Masao, Furuya Takeo, Nakamura Junichi, Eguchi Yawara, Suzuki Miyako, Kubota Go, Oikawa Yasuhiro, Sainoh Takeshi, Fujimoto Kazuki, Shiga Yasuhiro, Abe Koki, Kanamoto Hirohito, Inoue Masahiro, Kinoshita Hideyuki, Norimoto Masaki, Umimura Tomotaka, Takahashi Kazuhisa, Ohtori Seiji, Orita Sumihisa
Department of Orthopaedic Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
Department of Orthopaedic Surgery, Chiba Aoba Municipal Hospital, Chiba, Japan.
Spine Surg Relat Res. 2018 Jan 27;2(1):42-47. doi: 10.22603/ssrr.2017-0026. eCollection 2018.
Discogenic back pain remains poorly understood with respect to etiopathogenesis, despite being a considerable burden. We sought to examine the expression of vascular endothelial growth factor in injured intervertebral discs in rat caudal vertebrae.
Forty-eight male Sprague Dawley rats were assigned to 2 groups according to disc puncture injury: puncture (n = 32) or non-puncture (n = 16). Disc puncture was performed percutaneously such that the incision would be in the primary plane of motion for the coccygeal discs 5-6, 6-7, and 7-8. A 26-gauge needle was used to puncture each disc 10 times. Punctured discs were examined histologically by hematoxylin and eosin staining at 1, 7, 14, and 28 days post-injury.
Vascular endothelial growth factor was localized immunohistochemically, and determined quantitatively using an enzyme-linked immunosorbent assay. Peak inflammation occurred on the 7th day post-injury, but tissue degeneration continued until day 28. Local expression of vascular endothelial growth factor tended to be highest in the annulus fibrosus on the 7th and 14th days after puncture injury. The level of vascular endothelial growth factor was highest 1-day post-injury, and then gradually decreased thereafter. Furthermore, vascular endothelial growth factor levels in the puncture group were significantly higher than those in the non-puncture control group ( < 0.05).
We found increased expression of the inflammatory cytokine vascular endothelial growth factor in injured intervertebral discs, suggesting that vascular endothelial growth factor may be clinically important in discogenic back pain.
尽管椎间盘源性背痛是一个相当大的负担，但在病因发病机制方面仍未得到充分了解。我们试图研究大鼠尾椎损伤椎间盘内血管内皮生长因子的表达。
48只雄性Sprague Dawley大鼠根据椎间盘穿刺损伤分为2组：穿刺组（n = 32）或非穿刺组（n = 16）。经皮进行椎间盘穿刺，使切口位于尾椎椎间盘5-6、6-7和7-8的主要运动平面。使用26号针头对每个椎间盘穿刺10次。在损伤后1、7、14和28天，通过苏木精和伊红染色对穿刺后的椎间盘进行组织学检查。
采用免疫组织化学方法定位血管内皮生长因子，并使用酶联免疫吸附测定法定量测定。损伤后第7天出现炎症高峰，但组织退变持续至第28天。穿刺损伤后第7天和第14天，血管内皮生长因子在纤维环中的局部表达趋于最高。血管内皮生长因子水平在损伤后1天最高，此后逐渐下降。此外，穿刺组血管内皮生长因子水平显著高于非穿刺对照组（<0.05）。
我们发现损伤的椎间盘中炎症细胞因子血管内皮生长因子的表达增加，这表明血管内皮生长因子在椎间盘源性背痛中可能具有临床重要性。
