Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, Chile.
Botany and Zoology Department, Faculty of Science, University of Stellenbosch, Stellenbosch, South Africa.
Ecotoxicol Environ Saf. 2019 Nov 15;183:109573. doi: 10.1016/j.ecoenv.2019.109573. Epub 2019 Aug 20.
Aluminum (Al) bioaccumulation by a novel Al and drought tolerant Curtobacterium herbarum strain CAH5 isolated from rhizosphere soil of Beta vulgaris grown in acidic Andisols were examined. The rhizobacterial strain also presented important plant growth promoting traits even with Al and drought stresses under in-vitro conditions in broth. In experiments with a 2-6 mM as initial Al concentrations, the percentages of Al removal by bacteria were 89-93% and 78-91% within 72 h incubation under the normal and drought conditions, respectively. Cytogenotoxicity assay revealed that the toxicity of Al was reduced after bioaccumulation process. In the greenhouse study, formulated bio-inoculant CAH5 significantly improves the Lactuca sativa growth under Al and drought stress by reducing oxidative stress, lipid peroxidation and Al accumulation in plant parts. Our results highlighted that strain CAH5 could be used as a promising bioresource for restoration of agricultural soil with presence of phytotoxic Al improving crop production even under drought conditions.
从生长在酸性土壤中的饲用甜菜根际土壤中分离到一株新型耐铝和耐旱的短小杆菌 CAH5，研究了其对铝的生物积累作用。该根际细菌菌株在含有铝和干旱胁迫的液体培养基中，即使在体外条件下也表现出重要的促进植物生长的特性。在初始铝浓度为 2-6mM 的实验中，在正常和干旱条件下分别培养 72 小时后，细菌对铝的去除率分别为 89-93%和 78-91%。细胞遗传毒性试验表明，生物积累过程降低了铝的毒性。在温室研究中，配方生物接种剂 CAH5 通过降低植物各部分的氧化应激、脂质过氧化和铝积累，显著改善了生菜在铝和干旱胁迫下的生长。我们的研究结果表明，该菌株 CAH5 可作为一种有前途的生物资源，用于修复含有植物毒性铝的农业土壤，即使在干旱条件下也能提高作物产量。
