School of Pharmacy, University of Camerino, Camerino, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Environ Sci Pollut Res Int. 2019 Oct;26(29):30485-30497. doi: 10.1007/s11356-019-06217-4. Epub 2019 Aug 23.
Liriodendron tulipifera L. (Magnoliaceae), also known as "tulip tree," is a hardwood plant native to North America, cultivated all over the world and used on an industrial level, especially for its fine wood and to make honey. It has also been traditionally exploited for its antimalarial properties. However, our knowledge about the bioactivity of its essential oil remains patchy. In this research, we focused on the biological evaluation of the volatile fractions obtained from different parts of the plant which are normally discharged by industry, including leaves, flowers, and fruits. For the purpose, the essential oils were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Then, they were evaluated as radical scavenging, antioxidant, antimicrobial, and antiproliferative agents by using DPPH, ABTS, FRAP, disk diffusion, and MTT methods, respectively. The significant toxicity exhibited on human tumor cells, namely A375 malignant melanoma, HCT116 colon carcinoma, MDA-MB 231 breast adenocarcinoma, and T98G glioblastoma multiforme cell lines, prompted us to study the mechanism of action by acridine orange/ethidium bromide double staining and caspase 3 assays. Our findings shed light on the potential applications of tulip tree derivatives as anticancer drugs.
鹅掌楸（木兰科），又称“郁金香树”，是一种原产于北美的硬木植物，在世界各地被广泛种植并应用于工业领域，尤其是因其优质木材和可产蜂蜜而受到重视。它也一直被传统地用于治疗疟疾。然而，我们对其精油的生物活性的了解仍然很有限。在这项研究中，我们专注于研究从植物的不同部位（通常是工业排放的部位）获得的挥发性馏分的生物学评估，这些部位包括叶子、花朵和果实。为此，我们通过水蒸气蒸馏法获得精油，并通过气相色谱-质谱联用仪（GC-MS）进行分析。然后，我们分别使用 DPPH、ABTS、FRAP、圆盘扩散和 MTT 法评估它们作为自由基清除剂、抗氧化剂、抗菌剂和抗增殖剂的能力。这些精油对人肿瘤细胞（即恶性黑色素瘤 A375 细胞、结肠癌细胞 HCT116、乳腺癌 MDA-MB 231 细胞和多形性胶质母细胞瘤 T98G 细胞系）表现出显著的毒性，促使我们通过吖啶橙/溴化乙锭双重染色和 caspase 3 测定来研究其作用机制。我们的研究结果为鹅掌楸衍生物作为抗癌药物的潜在应用提供了依据。
