G-四链体结构及G-四链体相互作用化合物的核磁共振研究
NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.
作者信息
Lin Clement, Dickerhoff Jonathan, Yang Danzhou
机构信息
Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA.
Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA.
出版信息
Methods Mol Biol. 2019;2035:157-176. doi: 10.1007/978-1-4939-9666-7_9.
Abstract
G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.
摘要
G-四链体是在含有连续鸟嘌呤序列的区域形成的非经典四链核酸二级结构。这些G-四链体结构已被发现在具有生物学意义的核酸区域形成，包括人类端粒、基因启动子和mRNA的非翻译区。因此，它们被认为是有吸引力的治疗靶点。核磁共振（NMR）光谱是一种在生理相关条件下理解G-四链体结构及其与小分子相互作用的有力方法。在这里，我们介绍了我们研究小组用于研究生理相关溶液中DNA G-四链体结构及其配体相互作用的NMR方法。
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