Al-Suod Hossam, Ratiu Ileana-Andreea, Krakowska-Sieprawska Aneta, Lahuta Lesław, Górecki Ryszard, Buszewski Bogusław
Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Toruń, Poland.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
J Sep Sci. 2019 Oct;42(20):3243-3252. doi: 10.1002/jssc.201900539. Epub 2019 Sep 9.
The aim of the present study was to develop an optimization procedure for supercritical fluid extraction parameters, in order to obtain the highest possible yield of sugars and cyclitols from plant material. Response surface methodology based on Box-Behnken design was applied to evaluate the effect of: temperature (40, 60, 80°C), pressure (100, 200, 300 bar), and co-solvent (methanol) percentage (20, 25, 30%). As a result of the optimization process, we found that the highest amount of sugars (15.02 mg/gof dried material) and cyclitols (0.86 mg/g of dried material) was obtained when the following parameters were applied: 80°C, 228 bar, and 30% of methanol. Moreover, co-solvent concentration and temperature had a higher influence onto the obtained amounts compared with the pressure.
本研究的目的是开发一种超临界流体萃取参数的优化程序，以便从植物材料中获得尽可能高产量的糖类和环醇类。采用基于Box-Behnken设计的响应面法来评估温度（40、60、80°C）、压力（100、200、300 bar）和共溶剂（甲醇）百分比（20、25、30%）的影响。优化过程的结果表明，当采用以下参数时，获得了最高量的糖类（15.02 mg/g干物质）和环醇类（0.86 mg/g干物质）：80°C、228 bar和30%的甲醇。此外，与压力相比，共溶剂浓度和温度对所得产量的影响更大。
