Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, Spain.
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cadiz, Puerto Real, 11510 Cadiz, Spain.
Molecules. 2021 May 13;26(10):2884. doi: 10.3390/molecules26102884.
plants have been used in infusions and folk medicine for years for its diuretic and antiseptic properties and even for the treatment of infections. In addition, a recently published thorough study on this species has demonstrated its antioxidant, antibiotic, anti-inflammatory, anticarcinogenic and even antitumoral activities. These properties have been associated with the high content of anthocyanins in leaves and flowers. The aim of the present research is to optimize an ultrasound-assisted extraction methodology for the recovery of the anthocyanins present in flowers. For that purpose, a Box Behnken design with response surface methodology was employed, and the influence of four variables at different values was determined: namely, the composition of the extraction solvents (0-50% MeOH in water), the pH level of those solvents (3-7), the extraction temperature (10-70 °C), and the sample:solvent ratio (0.5 g:10 mL-0.5 g:20 mL). UHPLC-UV-vis has been employed to quantify the two major anthocyanins detected in the samples. The extraction optimum conditions for 0.5 g samples were: 20 mL of solvent (50% MeOH:HO) at 5 pH, with a 15 min extraction time at 70 °C. A precision study was performed and the intra-day and inter-day relative standard deviations (RSDs) obtained were 3.31% and 3.52%, respectively. The developed methodology has been successfully applied to other species to validate the suitability of the method for anthocyanin extraction.
多年来，植物一直被用于汤剂和民间医学中，因其具有利尿和防腐性能，甚至可用于治疗感染。此外，最近发表的一项关于该物种的彻底研究表明，它具有抗氧化、抗生素、抗炎、抗癌甚至抗肿瘤的活性。这些特性与叶子和花朵中高含量的花青素有关。本研究的目的是优化超声辅助提取方法，以回收花朵中存在的花青素。为此，采用 Box Behnken 设计和响应面法，确定了四个不同变量的影响：提取溶剂的组成（水中 0-50%甲醇）、这些溶剂的 pH 值（3-7）、提取温度（10-70°C）和样品与溶剂的比例（0.5 g:10 mL-0.5 g:20 mL）。UHPLC-UV-vis 用于定量分析样品中检测到的两种主要花青素。对于 0.5 g 样品的最佳提取条件为：50%甲醇:HO 溶剂 20 mL，pH 值为 5，提取时间为 15 分钟，温度为 70°C。进行了精密度研究，日内和日间相对标准偏差（RSD）分别为 3.31%和 3.52%。所开发的方法已成功应用于其他 物种，以验证该方法提取花青素的适用性。
