Supercritical Extraction Department, Sieć Badawcza Łukasiewicz - Instytut Nowych Syntez Chemicznych, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland.
Molecules. 2019 Sep 15;24(18):3354. doi: 10.3390/molecules24183354.
Due to their numerous health benefits associated with various diseases and anti-oxidation properties, the phenolic compounds collectively referred to as phytochemicals have attracted a lot of interest, however, a single extraction method for polyphenols has not been developed yet. Supercritical fluid extraction, a green extraction method, provides the final product without organic solvent residues. In this work the extraction of lavender was performed using supercritical carbon dioxide. A statistical experimental design based on the Box-Behnken (B-B) method was planned, and the extraction yields and total phenolic contents were measured for three different variables: pressure, temperature and extraction time. The ranges were 200-300 bar, 40-60 °C and 15-45 min. The extracts yields from scCO extraction were in the range of 4.3-9.2 wt.%. The highest yield (9.2 wt.%) was achieved at a temperature of 60 °C under the pressure of 250 bar after 45 min. It also corresponded to the highest total phenolic content (10.17 mg GAE/g extract). Based on the study, the statistically generated optimal extraction conditions to obtain the highest total phenolic compounds concentration from flowers of were a temperature of 54.5 °C, pressure of 297.9 bar, and the time of 45 min. Based on the scavenging activity percentage (AA%) of scCO extracts, it is concluded that the increase of extraction pressure had a positive influence on the increase of AA% values.
由于其与各种疾病相关的众多健康益处和抗氧化特性，被统称为植物化学物质的酚类化合物引起了广泛关注，然而，尚未开发出单一的多酚提取方法。超临界流体萃取作为一种绿色萃取方法，可提供无有机溶剂残留的最终产物。在这项工作中，使用超临界二氧化碳对薰衣草进行了提取。基于 Box-Behnken (B-B) 方法的统计实验设计被规划，并且针对三个不同变量：压力、温度和提取时间，测量了提取产率和总酚含量。范围是 200-300 巴、40-60°C 和 15-45 分钟。scCO 萃取的提取物产率在 4.3-9.2wt.%范围内。在 45 分钟后，在 250 巴的压力下，温度为 60°C 时获得了最高的产率（9.2wt.%）。它也对应于最高的总酚含量（10.17mgGAE/g 提取物）。根据研究，获得薰衣草花中总酚化合物浓度最高的统计生成的最佳提取条件是温度为 54.5°C、压力为 297.9 巴和时间为 45 分钟。根据 scCO 提取物的清除活性百分比（AA%）得出结论，增加提取压力对 AA%值的增加有积极影响。
