Department of Physiology and Pharmacology, Center for Basic Translational and Stroke Research, West Virginia University, Morgantown, WV, 26506, USA.
Department of Neuroscience, Center for Basic Translational and Stroke Research, West Virginia University, Morgantown, WV, 26506, USA.
Neuroimage. 2019 Nov 15;202:116109. doi: 10.1016/j.neuroimage.2019.116109. Epub 2019 Aug 22.
The cerebrovascular system provides crucial functions that maintain metabolic and homeostatic states of the brain. Despite its integral role of supporting cerebral viability, the topological organization of these networks remains largely uncharacterized. This void in our knowledge surmises entirely from current technological limitations that prevent the capturing of data through the entire depth of the brain. We report high-resolution reconstruction and analysis of the complete vascular network of the entire brain at the capillary level in adult female and male mice using a vascular corrosion cast procedure. Vascular network analysis of the whole brain revealed sex-related differences of vessel hierarchy. In addition, region-specific network analysis demonstrated different patterns of angioarchitecture between brain subregions and sex. Furthermore, our group is the first to provide a three-dimensional analysis of the angioarchitecture and network organization in a single reconstructed tomographic data set that encompasses all hierarchy of vessels in the brain of the adult mouse.
脑血管系统提供了维持大脑代谢和内稳态的关键功能。尽管它对支持大脑活力起着至关重要的作用，但这些网络的拓扑结构在很大程度上仍未得到描述。我们对这些网络的了解存在空白，完全是由于当前技术的限制，这些技术无法在整个大脑深度上获取数据。我们使用血管腐蚀铸型术报告了在成年雌性和雄性小鼠中以毛细血管水平重建和分析整个大脑完整血管网络的方法。全脑血管网络分析显示了性别相关的血管层次结构差异。此外，特定区域的网络分析显示了大脑子区域和性别之间的血管结构不同模式。此外，我们的团队首次提供了在单个重建的层析数据集中对血管结构和网络组织进行三维分析的方法，该数据集包含了成年小鼠大脑中所有血管层次的结构。
