Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco; Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, BP 2202, Fez, Morocco.
Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco; Department of Nephrology, University Hospital Hassan II, BP 1835, Atlas, Fez, Morocco.
J Integr Med. 2019 Nov;17(6):430-437. doi: 10.1016/j.joim.2019.08.001. Epub 2019 Aug 13.
The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L. leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate stones.
This survey was carried out using hydroalcoholic extract (E.FA) and infusion (I.FA) of A. unedo leaves. The quantification of phenolic compounds, flavonoids, flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultra-performance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry. Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and by the ferric reducing/antioxidant power (FRAP) assay. Litholytic activity of E.FA and I.FA was studied using a special model that resembles circuitry of the urinary system.
E.FA showed greater antioxidant efficacy than I.FA (P < 0.05). Its higher efficiency was shown via the values of median inhibitory concentration, which was close to (76.14 ± 0.91) µg/mL for E.FA versus (202.64 ± 5.77) μg/mL for I.FA using the DPPH method, and (53.77 ± 0.81) μg/mL for E.FA versus (236.86 ± 31.90) μg/mL for I.FA, using FRAP method. I.FA exhibited significantly higher litholytic activity compared to E.FA (P < 0.05), with dissolution values of 31.03% ± 0.63% versus 14.55% ± 0.65%, respectively.
Overall, the results suggest that the A. unedo is rich in bioactive compounds, and possesses antioxidant and litholitic abilities that are worthy of further study.
本研究旨在定量分析并鉴定欧洲杨梅叶中的生物活性化合物，以评估其抗氧化特性和对草酸钙结石的溶石活性。
本研究采用欧洲杨梅叶的水醇提取物（E.FA）和浸液（I.FA）进行研究。采用分光光度法测定酚类化合物、类黄酮、黄酮醇和花青素的含量，并通过超高效液相色谱-光电二极管阵列-电喷雾串联质谱联用技术对化学成分进行鉴定。采用 1,1-二苯基-2-苦基肼基（DPPH）法和铁还原/抗氧化能力（FRAP）测定法测定抗氧化活性。采用特殊模型研究 E.FA 和 I.FA 的溶石活性，该模型模拟泌尿系统的电路。
E.FA 的抗氧化效果优于 I.FA（P<0.05）。E.FA 的半数抑制浓度值更接近（76.14±0.91）μg/mL，表明其效率更高，而 I.FA 的半数抑制浓度值为（202.64±5.77）μg/mL，采用 DPPH 法测定；E.FA 的 FRAP 值为（53.77±0.81）μg/mL，而 I.FA 的 FRAP 值为（236.86±31.90）μg/mL，采用 FRAP 法测定。I.FA 的溶石活性明显高于 E.FA（P<0.05），溶解值分别为 31.03%±0.63%和 14.55%±0.65%。
总体而言，研究结果表明欧洲杨梅富含生物活性化合物，具有抗氧化和溶石能力，值得进一步研究。
