Moriya N, Nagaoki T, Okuda N, Taniguchi N
J Immunol. 1979 Oct;123(4):1795-8.
Unfractionated T lymphocytes from cord blood suppressed adult B cell differentiation into immunoglobulin-producing cells in pokeweed mitogen-stimulated co-culture system. Cord blood T cells were fractionated into T cells bearing Fc receptors for IgG (Tgamma cells) and T cells lacking Fc receptors for IgG(Tnon-gamma cells) by rosette formation with ox erythrocytes coated by the IgG fraction of rabbit antisera followed by Ficoll-Hypaque gradient sedimentation. T gamma cells from cord blood, even though isolated after the interaction with immune complexes, showed no suppressor activity on adult B cell differentiation, whereas Tnon-gamma cells exerted strong suppression to a similar extent to that by unfractionated cord T cells. The suppressor activity on B cell differentiation by Tnon-gamma cell as well as by unfractioned T cells from cord blood was completely abrogated by irradiation with 2000 rads. These results indicated that, contrary to suppressor function found in adult T cells, the suppressor activity in cord T cells might be exerted by a T cell subset lacking Fc receptors for IgG(Tnon-gamma cells).
在商陆丝裂原刺激的共培养系统中，来自脐血的未分离T淋巴细胞抑制成年B细胞分化为产生免疫球蛋白的细胞。通过用兔抗血清IgG部分包被的氧红细胞进行玫瑰花结形成，然后进行Ficoll-Hypaque梯度沉降，将脐血T细胞分为带有IgG Fc受体的T细胞（Tγ细胞）和缺乏IgG Fc受体的T细胞（T非γ细胞）。脐血中的Tγ细胞，即使在与免疫复合物相互作用后分离出来，对成年B细胞分化也没有抑制活性，而T非γ细胞则表现出与未分离的脐血T细胞相似程度的强烈抑制作用。脐血中T非γ细胞以及未分离的T细胞对B细胞分化的抑制活性在接受2000拉德照射后完全消除。这些结果表明，与成年T细胞中发现的抑制功能相反，脐血T细胞中的抑制活性可能由缺乏IgG Fc受体的T细胞亚群（T非γ细胞）发挥。
