Lueangjaroenkit Piyangkun, Teerapatsakul Churapa, Sakka Kazuo, Sakka Makiko, Kimura Tetsuya, Kunitake Emi, Chitradon Lerluck
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Laboratory of Applied Microbiology, Graduate School of Bioresources, Mie University, Tsu, Japan.
Mycobiology. 2019 Mar 31;47(2):217-229. doi: 10.1080/12298093.2019.1589900. eCollection 2019.
Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10-30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50 °C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.
从KU-RNW027中纯化出两种锰过氧化物酶（MnP1和MnP2）和一种漆酶（Lac1）。两种锰过氧化物酶在有机溶剂中均表现出高稳定性，且有机溶剂能激发它们的活性。金属离子在一定浓度下能激活两种锰过氧化物酶。在无氧化还原介质系统中，这两种锰过氧化物酶和漆酶在染料降解及药物失活方面发挥着重要作用。它们能在10 - 30分钟内完全降解雷马素亮蓝（25毫克/升），对雷马素藏青和雷马素亮黄表现出高降解活性，而漆酶能去除75%的雷马素红。这三种纯化酶能有效使四环素、强力霉素、阿莫西林和环丙沙星失活。最佳反应条件为50°C和pH 4.5。两种锰过氧化物酶能被有机溶剂和金属离子激活，这表明利用KU-RNW027对受有机溶剂和金属离子污染的环境中的芳香族化合物进行生物修复是有效的，且无需添加氧化还原介质。
