A.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, 119071 Moscow, Russia.
N.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 119334 Moscow, Russia.
Biomolecules. 2019 Aug 23;9(9):408. doi: 10.3390/biom9090408.
A comparative study of melanin and ommochrome-containing samples, isolated from the black soldier fly (BSF) by enzymatic hydrolysis, alkaline and acid alcohol extraction or by acid hydrolysis, was carried out. Melanin was isolated both as a melanin-chitin complex and as a water-soluble melanin. Acid hydrolysis followed by delipidization yielded a more concentrated melanin sample, the electron spin resonance (ESR) signal of which was 2.6 × 10 spin/g. The ommochromes were extracted from the BSF eyes with acid methanol. The antiradical activity of BSF melanins and ommochromes was determined by the method of quenching of luminol chemiluminescence. It has been shown that delipidization of water-soluble melanin increases its antioxidant properties. A comparison of the antioxidant activity of BSF melanins and ommochromes in relation to photoinduced lipid peroxidation was carried out. The ESR characteristics of native and oxidized melanins and ommochromes were studied. It is assumed that adult flies can be a useful source of natural pigments with antioxidant properties.
对通过酶解、碱性和酸性醇提取或酸解从黑水虻中分离得到的黑色素和类胡萝卜素含量样品进行了比较研究。黑色素既作为黑色素几丁质复合物，又作为水溶性黑色素被分离出来。酸水解后脱脂蛋白化得到更浓缩的黑色素样品，其电子自旋共振（ESR）信号为 2.6×10 自旋/g。类胡萝卜素从黑水虻眼睛中用酸性甲醇提取。通过淬灭鲁米诺化学发光法测定了黑水虻黑色素和类胡萝卜素的抗自由基活性。结果表明，水溶性黑色素的脱脂蛋白化可提高其抗氧化性能。比较了黑水虻黑色素和类胡萝卜素在光诱导脂质过氧化方面的抗氧化活性。研究了天然和氧化的黑色素和类胡萝卜素的 ESR 特征。假设成年蝇可以是具有抗氧化特性的天然色素的有用来源。
