使用鼠源cDNA探针定位68000道尔顿的人类神经丝基因（NF68）。

Localization of the 68,000-Da human neurofilament gene (NF68) using a murine cDNA probe.

作者信息

Somerville M J, McLachlan D R, Percy M E

机构信息

Department of Physiology, University of Toronto, Ont., Canada.

出版信息

Genome. 1988 Aug;30(4):499-500. doi: 10.1139/g88-083.

DOI:10.1139/g88-083

PMID:3145240

Abstract

A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have found secondary hybridization sites at the centromeric region of chromosome 2 and the long arm of chromosome 7, which are putative loci for other intermediate filaments.

摘要

最近一项使用人类基因组探针的研究表明，68000道尔顿神经丝基因（NF68）位于8号染色体短臂上。我们使用鼠源cDNA探针在65个中期染色体铺片上进行原位杂交，将人类NF68基因定位到8p21（20/370个银粒；P<0.0001）。此外，我们在2号染色体着丝粒区域和7号染色体长臂上发现了二级杂交位点，这些位点可能是其他中间丝的基因座。