Bulle F, Mattei M G, Siegrist S, Pawlak A, Passage E, Chobert M N, Laperche Y, Guellaën G
Hum Genet. 1987 Jul;76(3):283-6. doi: 10.1007/BF00283624.
We have determined the chromosomal location of the human gene for gamma-glutamyltransferase (GGT). This study was done by in situ hybridization of human metaphase spreads with a rat cDNA probe specific for this enzyme and constructed from two clones previously characterized in our laboratory. The final construct had a 1.6-kb-long insert covering 92% of the coding sequence for GGT. The new insert was also freed of any GC tails introduced for the cDNA cloning, because we observed that these sequences were responsible for a high background. Using this probe for the analysis of 136 human metaphase spreads, we observed a strong specific signal on chromosome 22 at the interface of q111-112 and a minor peak in q131. Thus GGT might represent a new marker for the study of certain diseases which have chromosomal abnormalities at these loci.
我们已经确定了人类γ-谷氨酰转移酶（GGT）基因的染色体定位。本研究通过用人中期染色体铺片与针对该酶的大鼠cDNA探针进行原位杂交来完成，该探针由我们实验室先前鉴定的两个克隆构建而成。最终构建体有一个1.6kb长的插入片段，覆盖了GGT编码序列的92%。新的插入片段也去除了为cDNA克隆引入的任何GC尾，因为我们观察到这些序列会导致高背景。使用该探针分析136个人类中期染色体铺片，我们在22号染色体q111-112交界处观察到一个强特异性信号，在q131处有一个小峰。因此，GGT可能代表一种新的标志物，用于研究在这些位点存在染色体异常的某些疾病。
