Handa T, Mitsuyama M, Serushago B A, Muramori K, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunology. 1988 Nov;65(3):427-32.
The effects of macrophage chemotactic factor (MCF) and macrophage-activating factor (MAF) on protection to Listeria monocytogenes were analysed using 'MCF-rich fraction' and murine recombinant interferon-gamma (rIFN-gamma). Recombinant IFN-gamma showed no macrophage chemotactic activity in the assays performed in vitro and in vivo. Although a single injection of either MCF-rich fraction or rIFN-gamma into the footpads of mice led to a significant degree of anti-listerial activity, the highest degree of protection was observed when injected together. The i.v. administration of 20,000 U rIFN-gamma did not raise significant protective activity against Listeria; however, injection with rIFN-gamma prior to that with the MCF-rich fraction into the footpad produced a higher level of protective activity than the group treated with MCF alone. In addition to MAF(IFN-gamma), MCF seems to play another important role in the full expression of protection against L. monocytogenes.
利用“富含巨噬细胞趋化因子（MCF）的组分”和小鼠重组干扰素-γ（rIFN-γ）分析了巨噬细胞趋化因子（MCF）和巨噬细胞激活因子（MAF）对单核细胞增生李斯特菌的保护作用。在体外和体内实验中，重组干扰素-γ均未表现出巨噬细胞趋化活性。虽然单独向小鼠足垫注射富含MCF的组分或rIFN-γ均可产生显著程度的抗李斯特菌活性，但二者联合注射时保护效果最佳。静脉注射20,000 U rIFN-γ对李斯特菌并未产生显著的保护活性；然而，先向足垫注射rIFN-γ，再注射富含MCF的组分，其产生的保护活性水平高于单独使用MCF处理的组。除了MAF（干扰素-γ）外，MCF似乎在针对单核细胞增生李斯特菌的保护作用的充分发挥中也起着重要作用。
