Schultz R M, Kleinschmidt W J
Nature. 1983;305(5931):239-40. doi: 10.1038/305239a0.
We have previously suggested that two lymphokine activities-macrophage activating factor (MAF) and gamma interferon (IFN-gamma)-are mediated by the same molecule. Striking similarities were noted in their cellular biosynthesis, rate of inactivation with acid treatment and heating, and elution profiles on Sephacryl S-200. In addition, highly specific polyclonal antibodies to murine IFN-gamma neutralized MAF and IFN-gamma to a similar degree. However, definitive studies require a pure product and we now report that murine IFN-gamma that had been cloned and expressed in a simian nonlymphoid cell line shows MAF activity. But it is not yet known whether IFN-gamma is responsible for all the MAF activity in media conditioned by T cells as the possibility for MAF heterogeneity remains.
我们之前曾提出，两种淋巴因子活性——巨噬细胞活化因子（MAF）和γ干扰素（IFN-γ）——由同一分子介导。在它们的细胞生物合成、酸处理和加热后的失活速率以及在Sephacryl S - 200上的洗脱图谱方面，发现了显著的相似性。此外，针对小鼠IFN-γ的高度特异性多克隆抗体对MAF和IFN-γ的中和程度相似。然而，确定性的研究需要纯产品，我们现在报告，在猿猴非淋巴细胞系中克隆并表达的小鼠IFN-γ具有MAF活性。但尚不清楚IFN-γ是否负责T细胞条件培养基中的所有MAF活性，因为MAF存在异质性的可能性仍然存在。
