Khamwut Ariya, Jevapatarakul Damita, Reamtong Onrapak, T-Thienprasert Nattanan Panjaworayan
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Oncol Lett. 2019 Sep;18(3):3128-3136. doi: 10.3892/ol.2019.10647. Epub 2019 Jul 22.
Vahl. is commonly consumed with the aim of curing cancer, inflammatory conditions and skin diseases in traditional Thai medicine. It is known to contain various phytochemicals; however, very little is known about the effects of protein hydrolysate on cancer cells, including its molecular mechanisms. The present study therefore investigated the anti-cancer activity of protein hydrolysates against epidermoid cancer of the skin cell line A431. Their effects on the apoptosis pathway and expression of proteins involved in the regulation of apoptosis, cell proliferation or cell cycle were also investigated. Crude extract of protein hydrolysate, partially purified peptides and purified peptides extracted from the aerial part of were administered to the A431 cells. The cytotoxicity effects were then determined using an MTT assay. As a result, protein hydrolysate significantly inhibited A431 cells with half inhibitory concentration equals to 425.9 ng protein/ml. By performing Annexin V assay, the partially purified peptides of were demonstrated to enhance the apoptosis pathway. Furthermore, western blot analysis revealed that the partially purified peptides of increased protein expression levels of RelA (p65) and Cyclin D1 proteins. However, did not increase the expression levels of p53-serine 15 phosphorylation (Ser15P).
在传统泰国医学中，人们通常食用Vahl.来治疗癌症、炎症和皮肤病。已知它含有多种植物化学物质；然而，关于蛋白质水解物对癌细胞的影响，包括其分子机制，人们了解甚少。因此，本研究调查了蛋白质水解物对皮肤表皮癌细胞系A431的抗癌活性。还研究了它们对凋亡途径以及参与凋亡调节、细胞增殖或细胞周期的蛋白质表达的影响。将从Vahl.地上部分提取的蛋白质水解物粗提物、部分纯化的肽和纯化的肽给予A431细胞。然后使用MTT法测定细胞毒性作用。结果，Vahl.蛋白质水解物显著抑制A431细胞，半数抑制浓度等于425.9 ng蛋白质/毫升。通过进行膜联蛋白V检测，证明Vahl.的部分纯化肽增强了凋亡途径。此外，蛋白质印迹分析显示，Vahl.的部分纯化肽增加了RelA（p65）和细胞周期蛋白D1蛋白的表达水平。然而，Vahl.并未增加p53丝氨酸15磷酸化（Ser15P）的表达水平。
