长链非编码RNA DGCR5通过下调miR-21抑制结肠癌细胞的增殖。
lncRNA DGCR5 inhibits the proliferation of colorectal cancer cells by downregulating miR-21.
作者信息
Huang Hansheng, Yang Xiaomei, Chen Jun, Fu Junmin, Chen Chaoting, Wen Jianmei, Mo Qiao
机构信息
Department of Medical Oncology, The Second Affiliated Hospital of Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, P.R. China.
Department of Pathology, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
出版信息
Oncol Lett. 2019 Sep;18(3):3331-3336. doi: 10.3892/ol.2019.10671. Epub 2019 Jul 26.
Long non-coding RNA (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) serves roles as a tumor suppressor or oncogene in different types of cancer. The current study aimed to explore the role of DGCR5 in colorectal cancer (CRC). It was revealed that the expression of DGCR5 was downregulated, while microRNA (miR)-21 was upregulated in CRC. The expression level of DGCR5 in tumor tissue decreased, while expression levels of miR-21 increased, with advancing stages of the disease. The expression levels of DGCR5 and miR-21 were inversely associated in tumor tissues. In CRC cells , miR-21 overexpression failed to significantly affect DGCR5, while DGCR5 overexpression resulted in reduced expression levels of miR-21. DGCR5 overexpression showed no significant effects on cancer cell migration and invasion, but suppressed cancer cell proliferation . miR-21 overexpression increased cancer cell proliferation and attenuated the effects of DGCR5 overexpression. Therefore, lncRNA DGCR5 may inhibit the proliferation of CRC cells by downregulating miR-21.
长链非编码RNA（lncRNA）迪乔治综合征关键区域基因5（DGCR5）在不同类型的癌症中发挥着肿瘤抑制因子或癌基因的作用。当前研究旨在探究DGCR5在结直肠癌（CRC）中的作用。结果显示，在结直肠癌中DGCR5的表达下调，而微小RNA（miR）-21的表达上调。随着疾病分期的推进，肿瘤组织中DGCR5的表达水平降低，而miR-21的表达水平升高。在肿瘤组织中，DGCR5和miR-21的表达水平呈负相关。在结直肠癌细胞中，miR-21过表达未能显著影响DGCR5，而DGCR5过表达导致miR-21的表达水平降低。DGCR5过表达对癌细胞的迁移和侵袭无显著影响，但抑制了癌细胞的增殖。miR-21过表达增加了癌细胞的增殖，并减弱了DGCR5过表达的作用。因此，lncRNA DGCR5可能通过下调miR-21来抑制结直肠癌细胞的增殖。
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