Li Junjie, Lin Peng, Tan Yuying, Cheng Ji-Xin
Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St, Boston, MA 02215, USA.
Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
Biomed Opt Express. 2019 Aug 1;10(8):4329-4339. doi: 10.1364/BOE.10.004329.
Thin tissue slice based histology has been used as a gold standard for disease diagnosis since over a hundred years ago. However, histopathological evaluation on two-dimensional slides suffers from large variations due to limited sampling. To improve the diagnostic accuracy, three-dimensional (3D) histology is performed through serial sectioning, staining, imaging and reconstruction of individual slices, which is highly time-consuming and labor intensive. We developed a volumetric stimulated Raman scattering (SRS) imaging method, which provides histology-like information in 3D context without the need for staining with dyes. Using a small molecule clearing agent, formamide, we performed tissue clearing within 30 min and achieved an imaging depth up to 500 µm in highly scattered tissues, including brain, kidney, liver and lung. Through a two-color SRS imaging scheme, we obtained histology-like images in cleared brain tissue slices. Our method has the potential for 3D tissue histopathology to improve the accuracy of histopathological examination.
自一百多年前以来，基于薄组织切片的组织学一直被用作疾病诊断的金标准。然而，由于取样有限，二维玻片上的组织病理学评估存在很大差异。为了提高诊断准确性，三维（3D）组织学通过对单个切片进行连续切片、染色、成像和重建来进行，这非常耗时且劳动强度大。我们开发了一种体积受激拉曼散射（SRS）成像方法，该方法无需用染料染色即可在三维环境中提供类似组织学的信息。使用小分子清除剂甲酰胺，我们在30分钟内完成了组织清除，并在包括脑、肾、肝和肺在内的高度散射组织中实现了高达500μm的成像深度。通过双色SRS成像方案，我们在清除后的脑组织切片中获得了类似组织学的图像。我们的方法有潜力用于三维组织病理学，以提高组织病理学检查的准确性。
