Arisov Mikhail Vladimirovich, Indyuhova Evgenia Nikolaevna, Arisova Gulnara Bakitovna
Ectoparasitoses laboratory, 28 Bolshaya, Cheremushkinskaya Street, Moscow 117218, Russia.
J Adv Vet Anim Res. 2018 Dec 28;6(1):25-32. doi: 10.5455/javar.2019.f308. eCollection 2019 Mar.
The object of the study was to examine the major pharmacokinetic parameters after a single application of a complex drug preparation for veterinary use based on fipronil, praziquantel, moxidectin, and pyriproxyfen in cats and dogs.
For dogs, the drug preparation was administered spot-on solution in the following dosage of active pharmaceutical substances: fipronil 27.0 mg/kg body weight (bwt), praziquantel 10.8 mg/kg bwt, moxidectin 6.75 mg/kg bwt, and pyriproxyfen 5.4 mg/kg bwt; for cats, the dosage was the following: fipronil 43.2 mg/kg bwt, praziquantel 17.28 mg/kg bwt, moxidectin 4.32 mg/kg bwt, and pyriproxyfen 8.64 mg/kg bwt. The blood samples were taken from dogs and cats. The principle of the method for determining praziquantel, trans-4-hydroxypraziquantel, pyriproxyfen, and fipronil in serum samples was chromatographed in a high-pressure liquid chromatograph with detection by means of a mass-spectrometric detector. The moxidectin content of the blood was detected by high-performance liquid chromatography.
The drug preparation active substances: praziquantel, fipronil, and moxidectin are absorbed into the blood of dogs and cats. The penetration of praziquantel into the systemic circulation and further into organs and tissues was proved. After topical administration, moxidectin is absorbed and distributed systemically and is slowly removed from the plasma, which manifests itself in detectable concentrations of moxidectin in the blood for 1 month.
The present results of pharmacokinetic investigations may promote to the determination of effective therapy strategy and prophylaxis of parasitic diseases in dogs and cats.
本研究的目的是检测一种基于氟虫腈、吡喹酮、莫昔克丁和吡丙醚的兽用复方制剂在猫和狗单次给药后的主要药代动力学参数。
对于狗，该药物制剂以如下活性药物成分剂量给予滴剂溶液：氟虫腈27.0毫克/千克体重，吡喹酮10.8毫克/千克体重，莫昔克丁6.75毫克/千克体重，吡丙醚5.4毫克/千克体重；对于猫，剂量如下：氟虫腈43.2毫克/千克体重，吡喹酮17.28毫克/千克体重，莫昔克丁4.32毫克/千克体重，吡丙醚8.64毫克/千克体重。从狗和猫身上采集血样。血清样品中吡喹酮、反式-4-羟基吡喹酮、吡丙醚和氟虫腈的测定方法原理是在配备质谱检测器的高压液相色谱仪中进行色谱分析。血液中莫昔克丁的含量通过高效液相色谱法检测。
该药物制剂的活性物质吡喹酮、氟虫腈和莫昔克丁被吸收进入狗和猫的血液。已证实吡喹酮可渗透到体循环中，并进一步渗透到器官和组织中。局部给药后，莫昔克丁被吸收并全身分布，且从血浆中缓慢清除，这表现为血液中莫昔克丁在1个月内可检测到浓度。
目前药代动力学研究结果可能有助于确定犬猫寄生虫病的有效治疗策略和预防措施。
