Sitther Viji, Tabatabai Behnam, Enitan Oluwatomisin, Dhekney Sadanand
Department of Biology, Morgan State University, Baltimore, MD 21251, USA.
Department of Plant Sciences, Sheridan Research and Extension Center, University of Wyoming, Sheridan, WY 82801, USA.
J Biol Methods. 2018 Jan 15;5(1):e83. doi: 10.14440/jbm.2018.208. eCollection 2018.
(. ), an oilseed species rich in poly-unsaturated fatty acids, has gained great importance as an industrial oil platform crop in recent years. Despite the potential benefits of for bioenergy applications, limited research has been conducted to improve its agronomic qualities. Hence, a simple and efficient technique for production of transgenic plants is warranted. In the present study, shoot apical meristems of two cultivars (Pl650159 and Pl650161) were transformed with strain 'EHA 105' harboring the enhanced green fluorescent protein (EGFP) and neomycin phosphotransferase II (nptII) genes. After two days of co-cultivation in the dark, explants were transferred to selection medium. Transgenic shoots were identified on the basis of green fluorescence and kanamycin resistance. Shoots were then rooted and transferred to potting mix soil for acclimatization. This protocol describes an efficient method to generate transgenic plants in as little as 4 weeks.
（.）是一种富含多不饱和脂肪酸的油籽作物，近年来作为一种工业用油平台作物变得极为重要。尽管（.）在生物能源应用方面有潜在益处，但为改善其农艺品质所开展的研究有限。因此，需要一种简单高效的技术来生产转基因（.）植物。在本研究中，用携带增强型绿色荧光蛋白（EGFP）和新霉素磷酸转移酶II（nptII）基因的‘EHA 105’菌株转化了两个（.）品种（Pl650159和Pl650161）的茎尖分生组织。在黑暗中共同培养两天后，将外植体转移到选择培养基上。根据绿色荧光和卡那霉素抗性鉴定转基因芽。然后将芽生根并转移到盆栽混合土中进行驯化。该方案描述了一种在短短4周内高效生成转基因（.）植物的方法。
