School of Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, Montréal, QC H9X3V9, Canada.
Rosell® Institute for Microbiome and Probiotics, 6100 Royalmount Avenue, Montréal, QC H4P 2R2, Canada.
Nutrients. 2019 Aug 26;11(9):2007. doi: 10.3390/nu11092007.
Probiotic supplementation plays a key role in maintaining intestinal homeostasis due to its ability to modulate gut microbiota. Although their potential as potent antioxidants have previously been explored, their ability to affect the redox status in the gut lumen of healthy subjects or those with gastrointestinal (GI) disorders remains unclear. In our study, we assessed the ability of single strain and multispecies probiotic supplementation to cause a change in the redox status of normal fecal water and in -infected fecal water using a simulated gastrointestinal model. Changes in redox status were assessed by ferric-reducing antioxidant power (FRAP), 2',2'-diphenyl-1-picrylhydrazyl (DPPH), and iron and copper chelation assays. The findings from our study showed that in normal fecal water, probiotic supplements, apart from ( R0011, showed a significant increase in iron chelation ( < 0.05), which was associated with lower FRAP and copper chelation. In -infected fecal water, all probiotic supplements showed a significant increase in FRAP ( < 0.05) and were associated with increased copper chelation. The DPPH assay showed no treatment effect in either fecal water. These findings suggest that mediates dysregulation of redox status, which is counteracted by probiotics through ferric-reducing ability and copper chelation.
益生菌补充剂通过调节肠道微生物群在维持肠道内环境稳定方面起着关键作用。尽管它们作为有效抗氧化剂的潜力以前已经得到了探索，但它们是否能够影响健康受试者或胃肠道（GI）疾病患者的肠道腔中的氧化还原状态仍不清楚。在我们的研究中，我们使用模拟胃肠道模型评估了单一菌株和多菌株益生菌补充剂对正常粪便水和感染粪便水氧化还原状态变化的影响。通过铁还原抗氧化能力（FRAP）、2'，2'-二苯基-1-苦基肼（DPPH）和铁和铜螯合测定来评估氧化还原状态的变化。我们的研究结果表明，在正常粪便水中，益生菌补充剂除了 （R0011）外，还显著增加了铁螯合作用（<0.05），这与 FRAP 和铜螯合作用降低有关。在感染粪便水中，所有益生菌补充剂均显著增加了 FRAP（<0.05），并与铜螯合作用增加有关。DPPH 测定在两种粪便水中均未显示出治疗效果。这些发现表明， 介导氧化还原状态的失调，而益生菌通过铁还原能力和铜螯合作用来对抗这种失调。
