Geertsema Sem, Jansen Bernadien H, van Goor Harry, Dijkstra Gerard, Faber Klaas Nico, Bourgonje Arno R
Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, 9713 GZ Groningen, The Netherlands.
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, 9713 GZ Groningen, The Netherlands.
Biomedicines. 2023 Nov 21;11(12):3107. doi: 10.3390/biomedicines11123107.
Oxidative stress is a key pathophysiological process associated with the development and progression of inflammatory bowel disease (IBD). Biomarkers for oxidative stress, however, are scarce, as are diagnostic tools that can interrogate an individual's gut redox status. This proof-of-concept study aimed to evaluate the potential utility of an oxidation-reduction potential (ORP) measurement probe, to quantify redox status in the feces of both patients with IBD and healthy controls. Previous studies using this ORP measurement probe demonstrated promising data when comparing ORP from severely malnourished individuals with that of healthy controls. To date, ORP analyses have not been performed in the context of IBD. We hypothesized that measuring the ORP of fecal water in patients with IBD might have diagnostic value. The current study, however, did not show significant differences in ORP measurement values between patients with IBD (median [IQR] 46.5 [33.0-61.2] mV) and healthy controls (25 [8.0-52.0] mV; = 0.221). Additionally, ORP measurements were highly unstable and rapidly fluctuated throughout time, with ORP values varying from +24 to +303 mV. Due to potential biological processes and limitations of the measuring equipment, this study was unable to reliably measure ORP. As a result, our findings indicate that ORP quantification may not be a suitable method for assessing fecal redox status and, therefore, does not currently support further exploration as a diagnostic or monitoring tool.
氧化应激是与炎症性肠病（IBD）的发生和发展相关的关键病理生理过程。然而，氧化应激的生物标志物稀缺，能够检测个体肠道氧化还原状态的诊断工具也同样匮乏。这项概念验证研究旨在评估氧化还原电位（ORP）测量探针在量化IBD患者和健康对照者粪便氧化还原状态方面的潜在效用。此前使用该ORP测量探针的研究在比较严重营养不良个体与健康对照者的ORP时取得了有前景的数据。迄今为止，尚未在IBD背景下进行ORP分析。我们假设测量IBD患者粪便水的ORP可能具有诊断价值。然而，当前研究并未显示IBD患者（中位数[四分位间距]46.5[33.0 - 61.2]mV）与健康对照者（25[8.0 - 52.0]mV；P = 0.221）的ORP测量值存在显著差异。此外，ORP测量极不稳定，且随时间快速波动，ORP值在+24至+303 mV之间变化。由于潜在的生物学过程以及测量设备的局限性，本研究无法可靠地测量ORP。因此，我们的研究结果表明，ORP量化可能不是评估粪便氧化还原状态的合适方法，故而目前不支持将其作为诊断或监测工具进行进一步探索。
