Poola Praveen Kumar, Afzal Muhammad Imran, Yoo Youngseung, Kim Ki Hean, Chung Euiheon
1Department of Biomedical Science and Engineering (BMSE), Gwangju Institute of Science and Technology (GIST), Gwangju, 61005 Republic of Korea.
4Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673 Republic of Korea.
Biomed Eng Lett. 2019 Jul 24;9(3):279-291. doi: 10.1007/s13534-019-00122-y. eCollection 2019 Aug.
Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples. Decoupling of the light sheet illumination from detection enables high-speed and large field-of-view imaging with minimal photobleaching and phototoxicity. These unique characteristics of the LSM technique can be easily adapted and potentially replace conventional histopathological procedures. In this review, we cover LSM technology from its inception to its most advanced technology; in particular, we highlight the human histopathological imaging applications to demonstrate LSM's rapid diagnostic ability in comparison with conventional histopathological procedures. We anticipate that the LSM technique can become a useful three-dimensional imaging tool for assessing human biopsies in the near future.
光片显微镜（Light sheet microscopy, LSM）是一种不断发展的光学成像技术，它采用平面照明进行光学切片和体积成像，应用范围涵盖细胞生物学、胚胎学和体内活体成像。在此，我们聚焦于LSM在组织样本方面新兴的生物医学应用。光片照明与检测的解耦实现了高速、大视野成像，同时光漂白和光毒性最小。LSM技术的这些独特特性易于应用，并且有可能取代传统的组织病理学程序。在本综述中，我们介绍了LSM技术从诞生到最先进技术的发展历程；特别是，我们着重介绍了其在人体组织病理学成像中的应用，以展示LSM与传统组织病理学程序相比的快速诊断能力。我们预计，在不久的将来，LSM技术能够成为评估人体活检样本的有用三维成像工具。
