溶血巴斯德氏菌T3血清型脂多糖O链的结构

Structure of the O-chain of the lipopolysaccharide of Pasteurella haemolytica serotype T3.

作者信息

Leitch R A, Richards J C

机构信息

Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.

出版信息

Biochem Cell Biol. 1988 Oct;66(10):1055-65. doi: 10.1139/o88-122.

DOI:10.1139/o88-122

PMID:3145754

Abstract

Cell-wall lipopolysaccharide isolated from Pasteurella haemolytica serotype T3 using the phenol-water extraction procedure was shown to be an S type lipopolysaccharide by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hydrolysis with mild acid afforded a lipid-free, antigenic O-chain polysaccharide. On the basis of one- and two-dimensional 1H and 13C nuclear magnetic resonance studies, in conjunction with microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a disaccharide repeating unit having the structure. [----3)-beta-D-G1cpNAc-(1----4)-alpha-L-Rhap-(1----]n

摘要

通过酚水提取法从溶血巴斯德菌T3血清型中分离出的细胞壁脂多糖，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示为S型脂多糖。用弱酸水解可得到无脂质的抗原性O链多糖。基于一维和二维1H和13C核磁共振研究，并结合微量分析化学方法，确定该O-多糖是具有结构[----3)-β-D-G1cpNAc-(1----4)-α-L-Rhap-(1----]n的二糖重复单元的线性聚合物。