溶血巴斯德氏菌A1血清型O-多糖的特性分析

Characterization of the O-polysaccharide of Pasteurella haemolytica serotype A1.

作者信息

Severn W B, Richards J C

机构信息

Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.

出版信息

Carbohydr Res. 1993 Feb 24;240:277-85. doi: 10.1016/0008-6215(93)84190-h.

DOI:10.1016/0008-6215(93)84190-h

PMID:7681360

Abstract

Lipopolysaccharide was isolated from Pasteurella haemolytica biotype A, serotype 1 by using the phenol-water extraction procedure. Hydrolysis with mild acid afforded a high-molecular-weight antigenic O-chain. On the basis of 1D and 2D NMR spectral studies and microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a trisaccharide repeating unit having the structure -->3)-beta-D-Galp-(1-->3)-beta-D-GalpNAc-(1-->4)-beta-D-Galp-(1--> This O-polysaccharide antigen is expressed by several P. haemolytica biotype A serotypes.

摘要

通过酚水提取法从溶血巴斯德氏菌生物型A 1型血清型中分离出脂多糖。用弱酸水解得到高分子量的抗原性O链。基于一维和二维核磁共振光谱研究以及微量分析化学方法，确定该O-多糖是具有结构为→3)-β-D-吡喃半乳糖-(1→3)-β-D-吡喃半乳糖胺-(1→4)-β-D-吡喃半乳糖-(1→的三糖重复单元的线性聚合物。这种O-多糖抗原由几种溶血巴斯德氏菌生物型A血清型表达。