Yoo Sanghyun, Dugasani Sreekantha Reddy, Chopade Prathamesh, Kesama Mallikarjuna Reddy, Gnapareddy Bramaramba, Park Sung Ha
Department of Physics and SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Republic of Korea.
ACS Omega. 2019 Apr 9;4(4):6530-6537. doi: 10.1021/acsomega.9b00319. eCollection 2019 Apr 30.
Researchers have begun to use DNA molecules as an efficient template for arrangement of multiple functionalized nanomaterials for specific target applications. In this research, we demonstrated a simple process to co-dope synthetic DNA nanostructures (by a substrate-assisted growth method) and natural salmon DNA thin films (by a drop-casting method) with divalent metal ions (M, e.g., Co and Cu) and trivalent lanthanide ions (Ln, e.g., Tb and Eu). To identify the relationship among the DNA and dopant ions, DNA nanostructures were constructed while varying the Ln concentration ([Ln]) at a fixed [M] with ion combinations of Co-Tb, Co-Eu, Cu-Tb, and Cu-Eu. Accordingly, we were able to estimate the critical [Ln] (named the optimum [Ln], [Ln]) at a given [M] in the DNA nanostructures that corresponds to the phase change of the DNA nanostructures from crystalline to amorphous. The phase of the DNA nanostructures stayed crystalline up to [Tb] ≡ 0.4 mM and [Eu] ≡ 0.4 mM for Co ([Tb] ≡ 0.6 mM and [Eu] ≡ 0.6 mM for Cu) and then changed to amorphous above 0.4 mM (0.6 mM). Consequently, phase diagrams of the four combinations of dopant ion pairs were created by analyzing the DNA lattice phases at given [M] and [Ln]. Interestingly, we observed extrema values of the measured physical quantities of DNA thin films near [Ln], where the maximum current, photoluminescence peak intensity, and minimum absorbance were obtained. M- and Ln-multidoped DNA nanostructures and DNA thin films may be utilized in the development of useful optoelectronic devices or sensors because of enhancement and contribution of multiple functionalities provided by M and Ln.
研究人员已开始将DNA分子用作一种高效模板，用于为特定目标应用排列多种功能化纳米材料。在本研究中，我们展示了一种简单的方法，通过底物辅助生长法将合成DNA纳米结构与二价金属离子（M，如Co和Cu）以及三价镧系离子（Ln，如Tb和Eu）共掺杂，通过滴铸法将天然鲑鱼DNA薄膜与二价金属离子和三价镧系离子共掺杂。为了确定DNA与掺杂离子之间的关系，构建了DNA纳米结构，同时在固定的[M]下改变Ln浓度（[Ln]），采用Co-Tb、Co-Eu、Cu-Tb和Cu-Eu的离子组合。因此，我们能够估计在给定[M]下DNA纳米结构中对应于DNA纳米结构从结晶相变为非晶相的临界[Ln]（称为最佳[Ln]，[Ln]opt）。对于Co，DNA纳米结构的相在[Tb]≡0.4 mM和[Eu]≡0.4 mM之前保持结晶（对于Cu，[Tb]≡0.6 mM和[Eu]≡0.6 mM），然后在高于0.4 mM（0.6 mM）时变为非晶相。因此，通过分析给定[M]和[Ln]下的DNA晶格相，创建了四种掺杂离子对组合的相图。有趣的是，我们在[Ln]opt附近观察到DNA薄膜测量物理量的极值，在该位置获得了最大电流、光致发光峰值强度和最小吸光度。由于M和Ln提供的多种功能的增强和贡献，M和Ln多掺杂的DNA纳米结构和DNA薄膜可用于开发有用的光电器件或传感器。
