Li Jun, Zhang Yuedong, Zou Zhen, Qing Zhihe, Yang Sheng, Yang Jianxiao, Zhang Lihua, Feng Feng, Yang Ronghua
School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, P. R. China.
College of Materials Science and Engineering, Hunan University, Changsha 410082, P. R. China.
ACS Omega. 2019 May 23;4(5):9074-9080. doi: 10.1021/acsomega.9b01009. eCollection 2019 May 31.
Fluorescent probes physisorbed on nanomaterials have emerged as a kind of useful and facile sensing platform for biological important molecules. However, nonspecific displacement in the physisorption systems is a non-negligible problem for the intracellular analysis. MIL (Materials of Institut Lavoisier), a subclass of metal-organic frameworks (MOFs), has high porosity, large surface area, and intriguing three-dimensional (3D) nanostructure with promising biological and biomedical applications such as molecular detection and drug delivery. Herein, we report MIL/aptamer-FAM as a nanosensor capable of resisting nonspecific displacement for intracellular adenosinetriphosphate (ATP) sensing and imaging. In this approach, by virtue of the remarkable quenching capability, high affinity of aptamers, and dramatic capability of resisting nonspecific displacement of 3D MIL-100, the assay and imaging of ATP in living cells were realized. Our results demonstrated that the MIL/aptamer-FAM nanosensor not only shows high selectivity for the detection of ATP in buffer but also is able to act as a "signal-on" nanosensor for specific imaging of ATP in living cells. The strategy reported here opens up a new way to develop MOF-based nanosensors for intracellular delivery and metabolite detection.
物理吸附在纳米材料上的荧光探针已成为一种用于检测生物重要分子的实用且便捷的传感平台。然而，对于细胞内分析而言，物理吸附系统中的非特异性置换是一个不可忽视的问题。金属有机框架材料（MOF）的一个子类——拉瓦锡研究所材料（MIL），具有高孔隙率、大表面积以及引人注目的三维（3D）纳米结构，在分子检测和药物递送等生物及生物医学应用方面颇具前景。在此，我们报道了MIL/适配体 - FAM作为一种能够抵抗非特异性置换的纳米传感器，用于细胞内三磷酸腺苷（ATP）的传感和成像。在这种方法中，凭借显著的猝灭能力、适配体的高亲和力以及3D MIL - 100卓越的抵抗非特异性置换能力，实现了活细胞中ATP的检测和成像。我们的结果表明，MIL/适配体 - FAM纳米传感器不仅在缓冲液中对ATP检测具有高选择性，而且能够作为一种“信号开启”纳米传感器用于活细胞中ATP的特异性成像。本文报道的策略为开发基于MOF的纳米传感器用于细胞内递送和代谢物检测开辟了一条新途径。
