Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-gu, Busan, 48513, South Korea.
World J Microbiol Biotechnol. 2019 Aug 28;35(9):141. doi: 10.1007/s11274-019-2718-6.
Chlorella vulgaris is an important freshwater alga that is widely used as a food source for humans and animals. High-salinity environments can cause accumulation of lipids and proteins in this species, but the mechanism of this accumulation and the salt response remain unclear. In this work, transcriptome analysis was performed for the C. vulgaris response to salt stress (1% and 3% NaCl) applied for different times (2 h and 4 h). In total, 5232 and 9196 were differentially expressed after 1% NaCl for 2 and 4 h, and 3968 and 9035 unigenes were differentially expressed after 3% NaCl for 2 and 4 h, respectively. The number of upregulated genes after 4 h of salinity stress was greater than the number of downregulated genes, suggesting that the alteration of gene expression may be related to a mechanism of adaptation to a high-salinity environment. Furthermore, gene ontology and KEGG pathway analyses revealed that numerous biological pathways are affected by salt stress. Among the upregulated pathways, the cytoplasmic calcium signaling pathway, which is involved in the regulation of homeostasis, was highly upregulated. Genes involved in the photosystem I light-harvesting pathway were downregulated under salt stress. These results provide foundational information on the effects of salt stress on C. vulgaris metabolism and its possible mechanism of surviving high concentrations of NaCl.
小球藻是一种重要的淡水藻类，被广泛用作人类和动物的食物来源。高盐环境会导致该物种积累脂质和蛋白质，但这种积累的机制和盐响应仍不清楚。在这项工作中，对小球藻对盐胁迫（1%和 3% NaCl）的反应进行了转录组分析，施加时间分别为 2 h 和 4 h。在 1% NaCl 处理 2 h 和 4 h 后，分别有 5232 和 9196 个基因差异表达，在 3% NaCl 处理 2 h 和 4 h 后，分别有 3968 和 9035 个基因差异表达。盐胁迫 4 h 后上调基因的数量大于下调基因的数量，表明基因表达的改变可能与适应高盐环境的机制有关。此外，GO 和 KEGG 通路分析表明，许多生物通路受到盐胁迫的影响。在上调的通路中，参与内稳态调节的细胞质钙信号通路高度上调。在盐胁迫下，参与光系统 I 光捕获途径的基因下调。这些结果为盐胁迫对小球藻代谢的影响及其在高浓度 NaCl 下生存的可能机制提供了基础信息。
