Mucilaginibacter limnophilus sp. nov., isolated from a lake.

A polyphasic taxonomy approach was used to characterize strain YBJ-36, isolated from a freshwater lake in Taiwan. Phylogenetic analyses, based on 16S rRNA gene sequences and coding sequences of an up-to-date bacterial core gene set (92 protein clusters), indicated that strain YBJ-36 formed a phylogenetic lineage in the genus Mucilaginibacter. 16S rRNA gene sequence similarity indicated that strain YBJ-36 is closely related to species within the genus Mucilaginibacter (93.8-97.8% sequence similarity) and is most similar to Mucilaginibacter fluminis TTM-2 (97.8%), followed by Mucilaginibacter roseus TTM-1 (97.2%). Microbiological analyses demonstrated that strain YBJ-36 is Gram-negative, aerobic, non-motile, rod-shaped, surrounded by a thick capsule, and forms pink-colored colonies. Strain YBJ-36 grew between 20-40°C (optimal range, 35-37°C), pH 5.5-7.0 (optimal pH of 6) and 0-2% NaCl (optimal concentration, 0.5%). The predominant fatty acids of strain YBJ-36 are iso-C and summed feature 3 (Cω7c and/or Cω6c), the major polar lipid is phosphatidylethanolamine, the major polyamine is homospermidine, and the major isoprenoid quinone is MK-7. The draft genome is approximately 4.63 Mb in size with a G+C content of 42.8 mol%. Strain YBJ-36 exhibited less than 35% DNA-DNA relatedness with Mucilaginibacter fluminis TTM-2 and Mucilaginibacter roseus TTM-1. Based on phenotypic and genotypic properties and phylogenetic inference, strain YBJ-36 should be classified in a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter limnophilus sp. nov. is proposed. The type strain is YBJ-36 (= BCRC 81056 = KCTC 52811 = LMG 30058).