Department of Chemistry, Xinzhou Teachers University, Xinzhou, Shanxi 034000, China.
Analyst. 2019 Oct 7;144(20):5965-5970. doi: 10.1039/c9an01326a.
Hydrogen peroxide (H2O2) plays crucial roles in immunological processes and has been revealed to be closely linked to the hypoxic-ischemic process. Thus, it is important to develop a reliable method for monitoring H2O2 in hypoxic-ischemic brain injury (HIBI). To achieve this, we report on a rationally designed fluorene-based ratiometric two-photon fluorescent probe for H2O2, i.e., FH2O2. The probe and fluorophore were tested under either one- or two-photon modes, respectively. After reacting with H2O2, the relative emission intensity ratio at wavelengths of 390-465 (Fblue) and 500-550 nm (Fyellow) of FH2O2 had a 6.5-fold increase (Fyellow/Fblue) within 40 min, and the maximal two-photon active cross-section value was detected as 66 GM at a wavelength of 750 nm. The probe exhibited high selectivity, low cytotoxicity, high sensitivity with a detection limit of 0.57 μM, and adequate photostability. After confirming satisfactory sensing performance of the probe in vitro, we were able to monitor H2O2in situ in mice with HIBI via two-photon microscopy, which could provide a potential method for clinical diagnosis during the neonatal HIBI process.
过氧化氢 (H2O2) 在免疫过程中发挥着关键作用，并且与缺氧缺血过程密切相关。因此，开发一种可靠的方法来监测缺氧缺血性脑损伤 (HIBI) 中的 H2O2 非常重要。为此，我们报告了一种基于芴的合理设计的用于 H2O2 的比率型双光子荧光探针，即 FH2O2。探针和荧光团分别在单光子或双光子模式下进行了测试。与 H2O2 反应后，FH2O2 在 390-465nm（Fblue）和 500-550nm（Fyellow）波长处的相对发射强度比在 40 分钟内增加了 6.5 倍（Fyellow/Fblue），并且在 750nm 处检测到最大双光子有效截面值为 66 GM。该探针具有高选择性、低细胞毒性、高灵敏度（检测限为 0.57μM）和足够的光稳定性。在体外确认探针具有令人满意的传感性能后，我们能够通过双光子显微镜在患有 HIBI 的小鼠体内原位监测 H2O2，这可为新生儿 HIBI 过程中的临床诊断提供一种潜在方法。
