Messina Marco S, Quargnali Gianluca, Chang Christopher J
Department of Chemistry and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, United States.
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States.
ACS Bio Med Chem Au. 2022 Dec 21;2(6):548-564. doi: 10.1021/acsbiomedchemau.2c00052. Epub 2022 Oct 11.
Activity-based sensing (ABS) offers a general approach that exploits chemical reactivity as a method for selective detection and manipulation of biological analytes. Here, we illustrate the value of this chemical platform to enable new biological discovery through a case study in the design and application of ABS reagents for studying hydrogen peroxide (HO), a major type of reactive oxygen species (ROS) that regulates a diverse array of vital cellular signaling processes to sustain life. Specifically, we summarize advances in the use of activity-based boronate probes for the detection of HO featuring high molecular selectivity over other ROS, with an emphasis on tailoring designs in chemical structure to promote new biological principles of redox signaling.
基于活性的传感（ABS）提供了一种通用方法，该方法利用化学反应性作为选择性检测和操纵生物分析物的手段。在此，我们通过一个案例研究来说明这个化学平台在实现新的生物学发现方面的价值，该案例研究涉及用于研究过氧化氢（HO）的ABS试剂的设计和应用，过氧化氢是活性氧（ROS）的一种主要类型，它调节各种重要的细胞信号传导过程以维持生命。具体而言，我们总结了基于活性的硼酸酯探针在检测HO方面的进展，该探针相对于其他ROS具有高分子选择性，重点在于调整化学结构设计以推动氧化还原信号传导的新生物学原理。
