非生物和生物胁迫下HSP70家族蛋白的全基因组分析及共表达分析

Genome-Wide Analysis of HSP70 Family Protein in and Coexpression Analysis Under Abiotic and Biotic Stress.

作者信息

Jasrotia Rahul Singh, Jaiswal Sarika, Yadav Pramod Kumar, Raza Mustafa, Iquebal Mir Asif, Rai Anil, Kumar Dinesh

机构信息

Centre for Agricultural Bioinformatics, ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India.

Department of Computational Biology & Bioinformatics, Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS), Allahabad, India.

出版信息

J Comput Biol. 2020 May;27(5):738-754. doi: 10.1089/cmb.2019.0166. Epub 2019 Aug 29.

DOI:10.1089/cmb.2019.0166

PMID:31464514

Abstract

Heat shock protein 70 (Hsp70), a 70-kDa protein, also known as a molecular chaperone, is highly conserved. It plays a major role in cellular functions such as protein folding, regulation of protein degradation, translocation of proteins across membranes, receptor signaling, and protein assembly or disassembly. is an important legume crop with available whole-genome sequence, but no such study on the HSP70 family is reported. A total of 32 HSP70s (Vr-HSP70s) were identified and described. They are phylogenetically clustered into four subgroups. Vr-HSP70s show variations in intron/exon organization. This indicates that introns may play an essential role in gene regulating. The coexpression analysis of Vr-HSP70s revealed that these genes were involved in both abiotic and biotic stresses. Three cytoplasmic hub genes namely Vr-HSP70-C-14, Vr-HSP70-C-29, and Vr-HSP70-C-30 were found common in both stresses. Our findings provide directions for future studies to dissect functional analysis of Vr-HSP70s in response to abiotic and biotic stresses.

摘要

热休克蛋白70（Hsp70）是一种70千道尔顿的蛋白质，也被称为分子伴侣，具有高度保守性。它在细胞功能中发挥着重要作用，如蛋白质折叠、蛋白质降解调控、蛋白质跨膜转运、受体信号传导以及蛋白质组装或拆卸。是一种具有可用全基因组序列的重要豆科作物，但尚未有关于HSP70家族的此类研究报道。共鉴定并描述了32个HSP70（Vr-HSP70s）。它们在系统发育上聚为四个亚组。Vr-HSP70s在内含子/外显子组织上存在差异。这表明内含子可能在基因调控中起重要作用。Vr-HSP70s的共表达分析表明，这些基因参与了非生物和生物胁迫。发现三个细胞质枢纽基因，即Vr-HSP70-C-14、Vr-HSP70-C-29和Vr-HSP70-C-30在两种胁迫中都很常见。我们的研究结果为未来剖析Vr-HSP70s在应对非生物和生物胁迫中的功能分析提供了方向。