Fan Chao, Yang Jie, Chen Rong, Liu Wei, Xiang Xu
Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Apr 25;40(4):1102-1119. doi: 10.13345/j.cjb.230450.
HSP70 protein, as an important member of the heat shock protein (HSP) family, plays an important role in plant growth, development, and response to biotic and abiotic stresses. In order to explore the role of gene family members in under low temperature, high temperature, drought, and salt stress, bioinformatics methods were used to identify the gene family members within the entire . genome. The expression of these genes under various abiotic stresses was then detected using quantitative real-time PCR (qRT-PCR). The results showed that the gene family consisted of 18 members, which were unevenly distributed across ten . chromosomes. The LcHSP70 protein contained 479-851 amino acids, with isoelectric points ranging from 5.07 to 6.95, and molecular weights from 52.44 kDa to 94.07 kDa. The predicted subcellular localization showed that LcHSP70 protein was present in the nucleus, cytoplasm, endoplasmic reticulum, mitochondria, and chloroplast. Phylogenetic analysis divided the LcHSP70 proteins into five subgroups, namely Ⅰ, Ⅱ, Ⅲ, Ⅳ, and Ⅵ. The promoter regions of the genes contained various -acting elements related to plant growth, development, hormone response, and stress response. Moreover, the expression of genes displayed distint tissue-specific expression level, categorized into universal expression and specific expression. From the selected 6 genes (i.e., , , , , , and ), their relative expression levels were assessed under different abiotic stresses using qRT-PCR. The results indicated that the gene family members exhibited diverse responses to low temperature, high temperature, drought, and salt stress, with significant variations in their expression levels across different time periods. These results provide a foundation for further exploration of the function of the gene family.
热休克蛋白70（HSP70）作为热休克蛋白（HSP）家族的重要成员，在植物生长、发育以及对生物和非生物胁迫的响应中发挥着重要作用。为了探究基因家族成员在低温、高温、干旱和盐胁迫下的作用，采用生物信息学方法在整个基因组中鉴定基因家族成员。然后使用定量实时PCR（qRT-PCR）检测这些基因在各种非生物胁迫下的表达情况。结果表明，基因家族由18个成员组成，它们不均匀地分布在十条染色体上。LcHSP70蛋白含有479 - 851个氨基酸，等电点范围为5.07至6.95，分子量为52.44 kDa至94.07 kDa。预测的亚细胞定位显示，LcHSP70蛋白存在于细胞核、细胞质、内质网、线粒体和叶绿体中。系统发育分析将LcHSP70蛋白分为五个亚组，即Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅵ。基因的启动子区域包含与植物生长、发育、激素响应和胁迫响应相关的各种顺式作用元件。此外，基因的表达表现出明显的组织特异性表达水平，分为普遍表达和特异性表达。从所选的6个基因（即、、、、、和）中，使用qRT-PCR评估它们在不同非生物胁迫下的相对表达水平。结果表明，基因家族成员对低温、高温、干旱和盐胁迫表现出不同的响应，其表达水平在不同时间段有显著变化。这些结果为进一步探究基因家族的功能提供了基础。
