Kholodilov N G, Bolshakov V N, Blinov V M, Solovyov V V, Zhimulev I F
Institute of Cytology and Genetics, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Chromosoma. 1988 Nov;97(3):247-53. doi: 10.1007/BF00292968.
Molecular and cytogenetic characteristics are given of a 2846 bp DNA sequence from the YDm12 clone, previously derived from the long arm of the Drosophila melanogaster Y chromosome. Sequence analysis revealed within it a 1176 bp fragment with 37 bp terminal inverted repeats, flanked by 6 bp direct repeats. This fragment (called "element 1360") appeared to be A-T rich, and was saturated with short direct and inverted repeats of different degrees of homology and consensus sequences for transcription, potential Z-DNA transition and autonomous replication. After in situ hybridization to polytene chromosomes, the element 1360 exhibited variable, strain-specifics location in the euchromatic parts of the chromosome arms, but constant heavy labelling of the X chromosome region 12E1-2, autosomal regions 42B1-3, 52A1-2, 62A1-2, 75B, 82C1-3, chromosome bases, the chromocentre and numerous sites of chromosome 4. The possible role of element 1360 in heterochromatin organization is discussed.
给出了来自YDm12克隆的一段2846 bp DNA序列的分子和细胞遗传学特征，该克隆先前源自黑腹果蝇Y染色体的长臂。序列分析显示其中有一个1176 bp的片段，带有37 bp的末端反向重复序列，两侧是6 bp的正向重复序列。这个片段（称为“元件1360”）似乎富含A-T，并且充满了不同程度同源性的短正向和反向重复序列以及转录、潜在Z-DNA转变和自主复制的共有序列。在与多线染色体进行原位杂交后，元件1360在染色体臂的常染色质部分表现出可变的、品系特异性的定位，但在X染色体区域12E1-2、常染色体区域42B1-3、52A1-2、62A1-2、75B、82C1-3、染色体基部、染色中心和4号染色体的许多位点持续出现重标记。讨论了元件1360在异染色质组织中的可能作用。
