Thomas D W, Bagg J, Walker D M
Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff.
J Oral Pathol. 1988 Sep;17(8):421-5. doi: 10.1111/j.1600-0714.1988.tb01308.x.
This study compared the in vitro cytotoxic activity against cultured NIH 3T3 mouse fibroblasts of peripheral blood leukocytes (PBL) from 13 patients with minor recurrent aphthous ulceration (RAU), 5 patients with non-aphthous oral ulceration and 23 age- and sex-matched healthy control subjects. Leukocytes from patients with RAU exerted a significantly greater degree of cytotoxicity towards the 3T3 cells than did those of control subjects. This enhanced cytotoxicity was abrogated by depletion of PBL bearing the CD5 antigen (T-lymphocytes). In contrast, PBL from patients with non-aphthous oral ulceration failed to exert an enhanced cytotoxic activity against 3T3 fibroblasts, when compared to PBL from healthy control subjects. It was concluded that PBL from patients with RAU exert a significantly enhanced cytotoxicity towards an unrelated xenogeneic target cell. This activity is a specific feature of RAU and in the assay system described appears to be mediated by T-lymphocytes.
本研究比较了13例轻型复发性阿弗他溃疡（RAU）患者、5例非阿弗他性口腔溃疡患者以及23例年龄和性别匹配的健康对照者外周血白细胞（PBL）对培养的NIH 3T3小鼠成纤维细胞的体外细胞毒活性。与对照者的白细胞相比，RAU患者的白细胞对3T3细胞表现出显著更强的细胞毒性。携带CD5抗原的PBL（T淋巴细胞）耗竭后，这种增强的细胞毒性被消除。相比之下，与健康对照者的PBL相比，非阿弗他性口腔溃疡患者的PBL对3T3成纤维细胞未表现出增强的细胞毒活性。得出的结论是，RAU患者的PBL对不相关的异种靶细胞表现出显著增强的细胞毒性。这种活性是RAU的一个特异性特征，在所描述的检测系统中似乎由T淋巴细胞介导。
