Reimer G, Steinkohl S, Djawari D, Hornstein O P
Br J Dermatol. 1982 Nov;107(5):529-36. doi: 10.1111/j.1365-2133.1982.tb00402.x.
The effect of blood lymphocytes on syngeneic oral epithelial cells was studied in twelve patients with Behçet's disease (BD) and twenty-one patients with recurrent aphthous ulcers (RAU). The control group consisted of twenty-five patients with non-aphthous skin disease and eight healthy individuals. Primary cell lines obtained from oral mucosa by suction blistering and subsequent enzymatic dissociation were used as target cells. Using a modified 51chromium release macro-assay, a significant lytic effect due to antibody-independent lymphocytotoxicity could be demonstrated in patients with BD, but no significant lysis of target cells was found in the RAU patients compared with the control group. These results provide further evidence that antibody-independent lymphocytotoxicity may play an important role in oral ulceration in BD.
在12例白塞病（BD）患者和21例复发性阿弗他溃疡（RAU）患者中研究了血液淋巴细胞对同基因口腔上皮细胞的作用。对照组由25例非阿弗他性皮肤病患者和8名健康个体组成。通过抽吸水疱并随后进行酶解从口腔黏膜获得的原代细胞系用作靶细胞。使用改良的51铬释放宏观测定法，可证明BD患者存在由抗体非依赖性淋巴细胞毒性引起的显著裂解作用，但与对照组相比，RAU患者未发现靶细胞有显著裂解。这些结果进一步证明抗体非依赖性淋巴细胞毒性可能在BD的口腔溃疡中起重要作用。
