Qi G R, Cao G J, Jiang P, Feng X L, Gu X R
Shanghai Institute of Biochemistry, Academia Sinica, China.
J Mol Evol. 1988;27(4):336-40. doi: 10.1007/BF02101196.
We have determined the complete sequences of 5S rRNAs from a lamprey (Lampetra reissneri), a lancelet (Branchiostoma belcheri), silkworms (Philosamia cynthia ricini, Bombyx mori, Antheraea pernyi), and a silkworm hybrid (artificially fertilized hybrid species of Philosamia cynthia ricini male x Bombyx mori female), as well as those of cotton seeds (Gossypium hirsutum L.). Having compared more than 170 eukaryotic 5S rRNAs of which seven sequences have been determined by our group as mentioned above, we have found that the "evolutionary sites" that exist at special locations in these structures are closely related to the evolution of eukaryotes. The changes proceed step by step in an orderly way, i.e., the change in nucleotide residues of the "evolutionary sites" depends on the order of the evolution of the species and shows group-specific patterns.
我们已经测定了七鳃鳗（雷氏七鳃鳗）、文昌鱼（白氏文昌鱼）、蚕（蓖麻蚕、家蚕、柞蚕）以及一种蚕杂交种（蓖麻蚕雄×家蚕雌人工授精杂交种）的5S rRNA完整序列，还有棉籽（陆地棉）的5S rRNA完整序列。在比较了170多种真核生物的5S rRNA后（其中七条序列如上文所述由我们小组测定），我们发现这些结构中特定位置存在的“进化位点”与真核生物的进化密切相关。这些变化以有序的方式逐步进行，即“进化位点”核苷酸残基的变化取决于物种的进化顺序，并呈现出特定群体的模式。
