Xian-Rong G, Nicoghosian K, Cedergren R J
Nucleic Acids Res. 1982 Sep 25;10(18):5711-6. doi: 10.1093/nar/10.18.5711.
The primary structure of 5S RNA isolated from the posterior silkgland of Philosamia cynthia ricini was determined using three in vitro labelling techniques. The derived sequence consists of 119 nucleotides and can be folded into the secondary structure model proposed for eukaryotic 5S RNAs. This 5S RNA differs from the Bombyx mori molecule in 9 positions and from the Drosophila melanogaster sequence in 14 positions. The comparison of evolutionary rates in insect 5S RNA with inferred rates in other eukaryotic phyla leads to the conclusion that 5S RNA evolution is not constant in different eukaryotic branches, a condition which must be taken into account in phylogenetic tree constructions.
利用三种体外标记技术确定了从蓖麻蚕后丝腺分离出的5S RNA的一级结构。推导的序列由119个核苷酸组成，可折叠成提出的真核生物5S RNA的二级结构模型。这种5S RNA与家蚕分子在9个位置不同，与黑腹果蝇序列在14个位置不同。将昆虫5S RNA的进化速率与其他真核生物门的推断速率进行比较，得出结论：5S RNA在不同真核生物分支中的进化并非恒定，这一情况在系统发育树构建中必须加以考虑。
