Department of Orthopaedics, Huaihe Hospital of Henan University , Kaifeng , Henan , China.
Department of Orthopaedics, The Second People's Hospital of Nanyang City , Nanyang , Henan , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3614-3620. doi: 10.1080/21691401.2019.1657877.
Osteoarthritis (OA) is one of the most characterized joint diseases associated with chondrocyte apoptosis. Juglanin has been reported to have anti-inflammation activity. This study aimed to evaluate the protective anti-inflammatory effects of juglanin in human OA chondrocytes. Human OA chondrocytes were pretreated with juglanin (10, 20 and 40 μM) for 2 h and subsequently stimulated with IL-1β for 24 h. Nitric oxide (NO) production was determined using the Griess method and prostaglandin E2 (PGE2), matrix metalloproteinase-3, -9 and -13 (MMP-3, MMP-9 and MMP-13), TNF-α, and IL-6 were assessed using ELISA. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-4 and -5 (ADAMTS-4 and ADAMTS-5) were detected by qRT-PCR and western blot analysis. NF-κB signalling molecules were detected by western blot analysis. The results showed that juglanin dose-dependently suppressed PGE2, NO, MMP-1, MMP3, MMP13, TNF-α and IL-6 production induced by IL-1β. The expression of COX-2, iNOS, ADAMTS-4 and ADAMTS-5 induced by IL-1β were also suppressed by juglanin pretreatment. Western blot analysis showed that juglanin suppressed IL-1β-induced NF-κB activation. Taken together, we found that juglanin inhibits IL-1β-induced inflammation through the regulation of NF-κB signalling. Juglanin might be used as a therapeutic agent for treating OA.
骨关节炎（OA）是与软骨细胞凋亡相关的最典型关节疾病之一。已报道胡桃醌具有抗炎活性。本研究旨在评估胡桃醌对人 OA 软骨细胞的保护抗炎作用。人 OA 软骨细胞用胡桃醌（10、20 和 40 μM）预处理 2 小时，然后用 IL-1β 刺激 24 小时。通过格里斯法测定一氧化氮（NO）的产生，通过 ELISA 测定前列腺素 E2（PGE2）、基质金属蛋白酶-3、-9 和 -13（MMP-3、MMP-9 和 MMP-13）、TNF-α 和 IL-6 的含量。通过 qRT-PCR 和 Western blot 分析检测诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、解整合素金属蛋白酶与凝血酶敏感素-4 和 -5（ADAMTS-4 和 ADAMTS-5）的表达。通过 Western blot 分析检测 NF-κB 信号分子。结果表明，胡桃醌呈剂量依赖性地抑制由 IL-1β 诱导的 PGE2、NO、MMP-1、MMP3、MMP13、TNF-α 和 IL-6 的产生。IL-1β 诱导的 COX-2、iNOS、ADAMTS-4 和 ADAMTS-5 的表达也被胡桃醌预处理所抑制。Western blot 分析表明，胡桃醌抑制了 IL-1β 诱导的 NF-κB 激活。综上所述，我们发现胡桃醌通过调节 NF-κB 信号通路抑制 IL-1β 诱导的炎症。胡桃醌可能被用作治疗 OA 的治疗剂。
