Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
University of Belgrade, Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
Food Funct. 2019 Sep 1;10(9):5939-5951. doi: 10.1039/c9fo01473j. Epub 2019 Aug 30.
In order to characterize and study the bioactivities of individual plant species and to determine how these characteristics are modified when preparing blends, five different plant species were selected: Erica australis L., Genista tridentata L., Melissa officinalis L., Mentha spicata L., and Prunella vulgaris L. Infusions prepared from each plant species and from three selected mixtures were analyzed in terms of nutritional values, phenolic compositions, and bioactive properties (antioxidant, antimicrobial, cytotoxic, and anti-inflammatory activities). The major compound detected in M. officinalis, M. spicata, and P. vulgaris infusions was rosmarinic acid, whilst in E. australis and G. tridentata flavonoid derivatives such as quercetin and genistein were identified. P. vulgaris and M. officinalis presented the best results in TBARS and OxHLIA assays, respectively. M. spicata and all mixtures presented anti-inflammatory activity. M. spicata showed the best cytotoxic properties and antimicrobial activity, and none of the infusions showed hepatotoxicity for non-tumour cells.
为了描述和研究各个植物物种的生物活性，并确定在制备混合物时这些特性如何发生变化，选择了五种不同的植物物种：南方越桔（Erica australis L.）、三齿猪殃殃（Genista tridentata L.）、普通薄荷（Mentha spicata L.）、柠檬香蜂草（ Melissa officinalis L.）和夏枯草（Prunella vulgaris L.）。从每个植物物种以及三种选定的混合物中制备的浸液，在营养价值、酚类成分和生物活性（抗氧化、抗菌、细胞毒性和抗炎活性）方面进行了分析。在薄荷、夏枯草和夏枯草浸液中检测到的主要化合物是迷迭香酸，而在南方越桔和三齿猪殃殃中则鉴定出了类黄酮衍生物，如槲皮素和染料木素。在 TBARS 和 OxHLIA 测定中，夏枯草和薄荷表现出最佳效果。夏枯草和所有混合物均表现出抗炎活性。夏枯草表现出最好的细胞毒性和抗菌活性，而且没有任何浸液对非肿瘤细胞显示出肝毒性。
