Molecular Systems Biology Laboratory, Department of Life Science, Chung-Ang University, Seoul, 06974, South Korea.
TheragenEtex Bio Institute, Suwon, 16229, South Korea.
Anal Biochem. 2019 Dec 1;586:113408. doi: 10.1016/j.ab.2019.113408. Epub 2019 Aug 27.
The increased use of high-throughput RNA-based analysis has spurred the demand for rapid and simple preparation of high quality RNA. RNA preparation from non-conventional yeasts having diverse cell wall and morphological characteristics is often inefficient using current methods adapted for the model yeast, Saccharomyces cerevisiae. We report a simple RNA preparation method based on glass bead-mediated breakage in a formamide/EDTA solution. High quality RNA is generated within 15 min from various non-conventional yeasts species. The obtained RNA can be directly used for experimentation without further RNA purification and buffer exchange.
高通量基于 RNA 的分析的使用增加，刺激了对快速简便地制备高质量 RNA 的需求。使用当前适用于模式酵母酿酒酵母（Saccharomyces cerevisiae）的方法，从具有不同细胞壁和形态特征的非常规酵母中制备 RNA 往往效率不高。我们报告了一种基于玻璃珠介导的在甲酰胺/EDTA 溶液中破裂的简单 RNA 制备方法。从各种非常规酵母物种中，在 15 分钟内即可生成高质量的 RNA。获得的 RNA 无需进一步的 RNA 纯化和缓冲液交换即可直接用于实验。
