苦参总生物碱和苦参碱抑制表皮葡萄球菌生物膜中的群体感应 2。
Total alkaloids of Sophora alopecuroides and matrine inhibit auto-inducer 2 in the biofilms of Staphylococcus epidermidis.
机构信息
Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Ningxia University, Yinchuan, Ningxia, 750021, China; Department of Medicine, Hetao College, Bayannaoer, Inner Mongolia, 015000, China.
Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Ningxia University, Yinchuan, Ningxia, 750021, China; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350000, China.
出版信息
Microb Pathog. 2019 Nov;136:103698. doi: 10.1016/j.micpath.2019.103698. Epub 2019 Aug 27.
Quorum sensing (QS) mediates the coordination of population-based behavior in bacteria, which is highly involved in the formation of bacterial biofilms and virulence of bacteria in vivo. Therefore, an inhibition of QS and biofilm growth is of therapeutic interest. This study exhibited the an auto-inducer molecule (AI-2) activity as the most important component of the QS system was positively correlated with the growth and biofilm formation of S. epidermidis strains. In addition, TASA and matrine have a capacity to inhibit AI-2 in three S. epidermidis strains compared to the control (p < 0.01). This result indicated TASA and matrine can also decrease AI-2 activity in the biofilm of S. epidermidis (p < 0.05). By comparison, TASA was more effective than ceftazidime and matrine to inhibit the AI-2 activity in biofilm S.epidermidis reference strain ATCC35984 (p < 0.05). This result indicated potentials of TCM compounds TASA and matrine in prevention of biofilm formation in Staphylococcus epidermidis infections.
群体感应（QS）介导细菌种群行为的协调，这与细菌生物膜的形成和体内细菌的毒力密切相关。因此，抑制 QS 和生物膜的生长具有治疗意义。本研究表明，作为 QS 系统最重要组成部分的自动诱导分子（AI-2）活性与表皮葡萄球菌菌株的生长和生物膜形成呈正相关。此外，与对照组相比，TASA 和苦参碱能够抑制三种表皮葡萄球菌菌株中的 AI-2（p<0.01）。这一结果表明 TASA 和苦参碱也可以降低表皮葡萄球菌生物膜中的 AI-2 活性（p<0.05）。相比之下，TASA 比头孢他啶和苦参碱更有效地抑制表皮葡萄球菌参考菌株 ATCC35984 生物膜中的 AI-2 活性（p<0.05）。这一结果表明 TCM 化合物 TASA 和苦参碱在预防表皮葡萄球菌感染生物膜形成方面具有潜力。
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