Beijing Key Lab of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China.
Shanghai Jahwa Corporation, Shanghai, China.
Exp Dermatol. 2019 Nov;28(11):1289-1297. doi: 10.1111/exd.14028. Epub 2019 Sep 16.
The microbiological basis of diaper dermatitis has not been clearly elucidated; however, a better understanding of microbial colonization may be vital for developing appropriate therapies.
Using 16S-rRNA gene sequencing technology, we characterized and compared the bacterial communities obtained from the buttock skin sites of children with diaper dermatitis and from healthy controls. Bacterial diversity in the buttock lesion area and subsequent recovery after emollient treatment have been discussed herein.
In buttock skin of children with or without diaper dermatitis, Staphylococcus and Anaerococcus were predominant in the total skin microbiome. Compared with the healthy group, the overall skin bacterial richness and diversity were higher in children with diaper dermatitis, with the abundance of Proteobacteria being significantly higher. In the diaper dermatitis group, the richness of Enterococcus, Erwinia and Pseudomonas was significantly higher, and the levels of Clostridium and Actinomyces were significantly lower than those in healthy children. Richness of Staphylococcus aureus was significantly higher in the diaper dermatitis group, whereas that of Staphylococcus epidermidis and Bifidobacterium longum was lower. Staphylococcus epidermidis and Staphylococcus haemolyticus, the dominant species found in buttock skin, were observed to recover earlier after the disease had improved through emollient treatment.
Staphylococcus epidermidis, as skin probiotic bacterium, and B longum, Clostridium butyricum and Lactobacillus ruminis, which are intestinal probiotic bacteria, are significantly decreased in diaper dermatitis lesions. These changes in the buttock skin microflora indicate an imbalance in the microflora and suggest that the intestinal microflora may be undergoing dynamic changes. The results of this study suggest that probiotic bacterial supplementation may be useful in the treatment and prevention of diaper dermatitis.
尿布皮炎的微生物基础尚未明确阐明；然而，更好地了解微生物定植对于开发适当的治疗方法可能至关重要。
使用 16S-rRNA 基因测序技术，我们对尿布皮炎患儿和健康对照者臀部皮肤部位的细菌群落进行了特征描述和比较。本文讨论了臀部病变区域的细菌多样性以及润肤剂治疗后的后续恢复情况。
在患有或未患有尿布皮炎的儿童的臀部皮肤中，葡萄球菌属和厌氧球菌属是皮肤微生物群的主要菌群。与健康组相比，尿布皮炎患儿的皮肤总细菌丰富度和多样性更高，变形菌门的丰度明显更高。在尿布皮炎组中，肠球菌属、欧文氏菌属和假单胞菌属的丰富度明显更高，而梭菌属和放线菌属的水平明显低于健康儿童。金黄色葡萄球菌属的丰富度在尿布皮炎组中明显更高，而表皮葡萄球菌属和长双歧杆菌属的丰富度较低。在疾病通过润肤剂治疗得到改善后，发现臀部皮肤中占主导地位的表皮葡萄球菌属和表皮葡萄球菌属和溶血葡萄球菌属恢复得更早。
作为皮肤益生菌的表皮葡萄球菌属和双歧杆菌属、丁酸梭菌属和鼠李糖乳杆菌属，这些都是肠道益生菌，在尿布皮炎病变中显著减少。这些臀部皮肤微生物群的变化表明微生物群失衡，并提示肠道微生物群可能正在发生动态变化。本研究结果表明，益生菌细菌补充可能对尿布皮炎的治疗和预防有用。
