Department of Genetics and Microbiology, Faculty of Science, Charles University, Albertov 6, 128 00 Prague 2, Czech Republic.
Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University, V Úvalu 84, 15006 Prague 5, Czech Republic.
J Microbiol Methods. 2019 Dec;167:105705. doi: 10.1016/j.mimet.2019.105705. Epub 2019 Aug 28.
Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance. The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et al., 2017) to detect persistent or tolerant bacterial cells in clinical isolates of Staphylococcus aureus. The principle of the test is to resuscitate the subpopulation of persistent or tolerant bacterial cells following a disk diffusion test by glucose. Results of the TDtest were evaluated using time killing experiments for three pairs of consecutive S. aureus isolates from lower respiratory airway samples of three cystic fibrosis patients with chronic staphylococcal infections. TDtest enabled semi-quantitative detection of persistent or tolerant bacterial populations in all analyzed isolates for oxacillin, vancomycin, and ciprofloxacin to which isolates studied were susceptible. Therefore, TDtest is a promising method for rapidly determining persistence/tolerance in clinical isolates of S. aureus.
除了天然和获得性耐药机制外，细菌还可以通过持久性或耐受性等复杂机制来应对抗生素的存在。本研究的主要目的是评估新开发的耐量盘试验（TDtest）（Gefen 等人，2017 年）是否适合检测金黄色葡萄球菌临床分离株中的持久性或耐量细菌细胞。该试验的原理是通过葡萄糖使扩散试验中存活的亚群细菌复活。使用来自三名囊性纤维化患者下呼吸道样本中连续三对金黄色葡萄球菌分离株的时间杀伤实验来评估 TDtest 的结果，这些患者患有慢性金黄色葡萄球菌感染。TDtest 能够对半定量检测所有分析分离物中对耐甲氧西林金黄色葡萄球菌、万古霉素和环丙沙星敏感的持久性或耐量细菌群体。因此，TDtest 是一种很有前途的方法，可用于快速确定金黄色葡萄球菌临床分离株中的持久性/耐量。
