Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan; Miroku Medical Laboratory Inc., 659-2 Innai, Saku, Nagano 384-2201, Japan.
J Glob Antimicrob Resist. 2020 Mar;20:253-259. doi: 10.1016/j.jgar.2019.08.016. Epub 2019 Aug 28.
To characterise the genotypic profiles of methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates from companion animals and to investigate their association with those from humans in Japan.
Non-duplicated MRSA clinical isolates recovered between July 2016 and January 2018 were analysed. The MRSA isolates were typed by polymerase chain reaction (PCR)-based open reading frame (ORF) typing (POT) scores, SCCmec types, multilocus sequence typing, and virulence gene profiles. Phylogenetic comparison of those isolates with previously described human isolates was performed.
Among 56 MRSA isolates (33 cats, 20 dogs and three rabbits), 26 isolates with a POT1 score of 93, SCCmec type II mostly belonged to CC5, including ST5. Twenty-six isolates with a POT1 score of 106, SCCmec type IV showed diversity of STs: 15 isolates belonged to CC8, mainly including ST8, and 11 isolates belonged to CC1, including ST1 and newly identified STs 4768, 4775, and 4779. Two cat isolates were ST8-SCCmec type IV possessing pvl/ACME-arcA, presumed to be the hypervirulent community-associated MRSA (CA-MRSA) clone USA300. Notably, all three rabbit isolates belonged to ST4768. The POT1 score 106 CA-MRSA isolates from animals and humans were divided into two large clusters of CC1 and CC8, where host species-specific sub-clusters were not identified within each cluster. A large cluster of POT1 score 93 healthcare-associated MRSA (HA-MRSA) isolates from animals and humans consisted of sub-clusters formed exclusively by the vast majority of human isolates and those formed by animal and human isolates.
Companion animals could be potential reservoirs and vehicles for the transmission of CA-MRSA to humans, and could transmit companion animal-adaptive HA-MRSA lineages to humans as their second reservoirs.
分析来自日本宠物的耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株的基因型特征，并探讨其与人类分离株的相关性。
分析了 2016 年 7 月至 2018 年 1 月期间分离的非重复耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株。采用基于聚合酶链反应（PCR）的开放阅读框（ORF）分型（POT）评分、SCCmec 型、多位点序列分型和毒力基因谱对 MRSA 分离株进行分型。对这些分离株与先前描述的人类分离株进行了系统发育比较。
在 56 株 MRSA 分离株（33 株猫、20 株狗和 3 只兔）中，26 株 POT1 评分 93、SCCmec 型 II 的分离株主要属于 CC5，包括 ST5。26 株 POT1 评分 106、SCCmec 型 IV 的分离株显示出 ST 多样性：15 株分离株属于 CC8，主要包括 ST8，11 株分离株属于 CC1，包括 ST1 和新鉴定的 ST4768、4775 和 4779。2 株猫分离株为 ST8-SCCmec 型 IV 携带 pvl/ACME-arcA，被认为是高毒力社区相关耐甲氧西林金黄色葡萄球菌（CA-MRSA）克隆 USA300。值得注意的是，所有 3 只兔分离株均属于 ST4768。来自动物和人类的 POT1 评分 106 CA-MRSA 分离株分为 CC1 和 CC8 两个大簇，在每个簇内未鉴定到宿主种特异性亚簇。来自动物和人类的 POT1 评分 93 株医源性耐甲氧西林金黄色葡萄球菌（HA-MRSA）分离株的大簇由完全由大多数人类分离株形成的亚簇和由动物和人类分离株形成的亚簇组成。
宠物可能是 CA-MRSA 向人类传播的潜在储主和传播媒介，并且可能作为人类的第二个储主，将宠物适应的 HA-MRSA 株系传播给人类。
